GAPDH-2 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

GAPDH-2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human GAPDH-2
  Glu75-Ala222
  Accession # O14556
• Specificity: Detects human GAPDH-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GAPDH and recombinant mouse GAPDH is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAPDH-2 Antibody [Alexa Fluor® 405]

• EC 1.2.1
• EC 1.2.1.12
• GAPD2
• GAPD2HSD-35
• GAPDH2
• GAPDH-2
• GAPDH-2glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• GAPDHS
• GAPDS
• glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• HSD-35
• Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
• Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Background

GAPDH-2 (Glyceraldehyde-3-phosphate dehydrogenase 2; also GAPD2 and GAPDS) is a 40-44 kDa member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56 kDa in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408 amino acids (aa) in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa 223-226. One splice variant shows a nine aa insertion after Ser220. Over aa 75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.