GAPDH-2 Antibody (Hs-8) - BSA Free

Flow Cytometry: GAPDH-2 Antibody (Hs-8) [NB500-456] - Detection of GAPDHS in acrosomes of human sperms with anti-GAPDHS (Hs-8) FITC.

Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in Analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 ug/ml) GAM APC.

• Reactivity: Hu, Po
• Applications: WB, Flow, ICC/IF
• Clone: Hs-8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GAPDH-2 Antibody (Hs-8) - BSA Free Summary

• Description: Novus Biologicals Mouse GAPDH-2 Antibody (Hs-8) - BSA Free (NB500-456) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This GAPDH-2 antibody was developed against the full length human protein extracted from sperms.
• Specificity: The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, previously identified under the general name "intra-acrosomal protein".
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAPDHS
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 3-12 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 1:1000
• Application Notes: Western Blot Detects bands of approximately 220, 50, 34 and 21 kDa. Immunocytochemistry: Staining technique: Membrane permeabilization (acetone) is essential.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris buffered saline (TBS), pH 8.0
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Multi-step

Alternate Names for GAPDH-2 Antibody (Hs-8) - BSA Free

• EC 1.2.1
• EC 1.2.1.12
• GAPD2
• GAPD2HSD-35
• GAPDH2
• GAPDH-2
• GAPDH-2glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• GAPDHS
• GAPDS
• glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• HSD-35
• Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
• Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Background

This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAPDHS
• Entrez:
  • 56(Human)