GAP-43 [p Ser41] Antibody - Azide Free

Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Av-Bi, Pm, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

GAP-43 [p Ser41] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit GAP-43 [p Ser41] Antibody - Azide Free (NB300-179) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH. Accession # P07936
• Modification: p Ser41
• Localization: Growth cone
• Marker: Neuronal Marker
• Specificity: Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Primate (100%), Xenopus (100%), Bovine (100%), Finch (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAP43
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: NB300-179 can be used in Western blot where a band is seen at ~ 43 kDa, representing phosphorylated Gap43. In some tissues the antibody also recognizes a lower MW band that is also recognized by the PAN Gap43 antibody and, thus, it is likely to be a breakdown product. Immunohistochemical analysis has not yet been determined.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for GAP-43 [p Ser41] Antibody - Azide Free

• Axonal membrane protein GAP-43
• B-50
• Basp2
• calmodulin-binding protein P-57
• GAP43
• GAP-43
• growth associated protein 43
• Growth-associated protein 43
• nerve growth-related peptide GAP43
• Neural phosphoprotein B-50
• Neuromodulin
• neuron growth-associated protein 43
• PP46
• protein F1

Background

GAP-43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAP43
• Entrez:
  • 14432(Mouse)