GAP-43 Antibody - BSA Free

Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Review image from confirmed customer on mouse E15.5 paraffin sections.

Western Blot: GAP-43 Antibody [NB300-143] - Different tissue and cell lysates using rabbit pAb to GAP43, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein

Immunohistochemistry: GAP-43 Antibody [NB300-143] - Representative images of immunohistological staining (brown) of OMP-positive (OMP+) cells, GAP43+ immature ORNs. Tissue sections were counterstained with the nuclear dye hematoxylin (blue). Numbers of SOX2+ ORN progenitors and Ki67+ actively proliferating cells per mm of the basal layer and OMP+ mature ORNs, GAP43+ immature ORNs, and Cas3+ apoptotic cells per mm of the OE in saline or rhIGF-1-treated mice. Open circles, rectangles, and triangles represent the values for each mouse in the saline, low-IGF-1, and high-IGF-1 treated groups (each n = 6), respectively. Image collected and cropped by Citeab from the following publication (Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium. Front Aging Neurosci (2018)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GAP-43 Antibody [NB300-143] - Posterior MOE sections at approximately the same region where EOG was recorded were obtained from control and Skn-1a-/- mice exposed to either water or chemicals for 2 wks. The sections were immunoreacted with antibodies against GAP43 and OMP and stained with DAPI. Confocal images were taken from olfactory turbinate II. Similar morphology and marker expression were found in four groups of mice, indicating there was no obvious tissue damage in both control and Skn-1a-/- mice after chemical exposure in the posterior MOE regions where EOG recordings were performed. Scale bar: 50 um. Image collected and cropped by CiteAb from the following publication (//eneuro.sfn.org/lookup/doi/10.1523/ENEURO.0135-17.2017), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Rat E18 mixed neuron/glia cultures with rabbit GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).

Western Blot: GAP-43 Antibody [NB300-143] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP-43 . A prominent band running at ~43kDa represents the full length GAP-43.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NB300-143] - Mixed neuron-glial cultures stained with RPCA-GAP43 (red), blue is DNA staining.

Western Blot: GAP-43 Antibody [NB300-143] - Silencing TFAP4 induces neuroblastoma cell differentiation. a Morphology of neuroblastoma cells after silencing TFAP4. Cells were grown in culture for 8 days, with 1 μg/ml doxycycline (shRNA induced) or without doxycycline. b Neurites # per field, & neurite length (pixels) were quantified with or without doxycycline. Mean ± std dev. *, P < 0.05; **, P < 0.01, ****, P < 0.0001. cGAP43 expression level (qRT-PCR) & protein level (western blot) in neuroblastoma cells. Gene expression was measured by quantitative PCR 4 days after induction of shRNA against TFAP4. Protein samples for western blot were collected from MYCN-amplified cells that were cultured with or without doxycycline (1 μg/ml) for 6 days Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29880876), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - LAMP1 increases in SDC-null corneas 14 days after trephine wound. Representative 3D images rotated to generate cross-sectional views show the localization of GAP43 (green), beta III tubulin (red), & LAMP1 (blue) in WT (top) & SDC1-null (bottom) corneas. These images show staining within a 0.5-μm cross-sections. Data indicate that axon fragments are being degraded (arrows). Representative INTs (*) & SBNs (#) have been highlighted & enlarged 3-fold. When LAMP1 expression within apical & basal cell layers 14 days after trephine injury is quantified & compared with unwounded controls (Fig. 2B), LAMP1 increases over 3-fold in SDC1-null corneas but remains the same in WT corneas. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28973369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAP-43 Antibody [NB300-143] - Obesity & diabetes led to white adipose tissue neuropathy.The BTBR ob/ob (MUT) model of obesity & diabetes was compared to BTBR +/+ wild-type (WT) for body weight measurements (a), & adiposity (b). Body & tissue weight data were analyzed by two-tailed Student’s t-test. Von Frey tactile allodynia analysis was performed on MUT & WT animals to determine onset of peripheral neuropathy (c). Von Frey data was analyzed by ANOVA with Sidak’s post hoc test. For (a-c), all males; WT N = 8, 12–20 weeks old; MUT N = 6, 12–24 weeks old. Protein levels of PGP9.5 (d), as well as TH (e) in inguinal scWAT of the MUT & WT mice were measured by western blotting. For (e), lane 5 was excluded from analyses due to uneven resolution of housekeeper. For (d-e), all males; WT N = 5, 12–20 weeks old & MUT N = 4, 12–20 weeks old. Protein expression of PGP9.5 (f), TH (g), PSD95 (h), & GAP43 (i) in inguinal scWAT of 12–25 weeks old WT, 12 week old MUT & 24–28 week old MUT was measured by western blotting. All males, N = 3 per group. Western blot data were normalized to either beta -Tubulin or Cyclophilin B, band intensity were quantified in Image J, & analyzed by two-tailed Student’s t-test. Error bars are SEMs. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31509546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Intraepithelial nerve terminals extension into the apical most cell layers increases in SDC1-null corneas after trephine injury. Representative 3D images rotated to generate cross-sectional views show the localization of beta III tubulin (red) & GAP43 (green) at 4, 14, & 28 days after trephine wounding in WT & SDC1-null corneas. Punctate GAP43 is increased within the epithelium in SDC1-null compared with WT corneas at 14 days (*). beta III tubulin+ & GAP43+ INTs in the apical most cell layers were quantified as a function of time after trephine injury & normalized relative to control (presented in Fig. 2A). Asterisks within bars indicate differences that are significant relative to unwounded controls; asterisks between bars indicate significant differences between time points. In WT corneas, the numbers of beta III tubulin+ & GAP43+ INTs extending apically decrease relative to controls at 28 days for beta III tubulin+ INTs & at 4 & 28 days for GAP43+ INTS, in SDC1-null corneas, both beta III tubulin+ & GAP43+ INTs increase at all time points assessed. Scale bar: 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28973369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NB300-143] - Differences in INTs & LAMP1 localization in WT & SDC1-null adult corneas. Representative high-resolution confocal 3D images rotated as indicated in the cartoon to the left were used to generate cross sectional views to show the localization of beta III tubulin (red) & GAP43 (green) in intraepithelial nerve terminals in control WT & SDC1-null corneas. The images shown in (A) project through 135 μm of tissue, images in (B) project through 0.5 μm of tissue. (A) Intraepithelial corneal nerves are shown to be linear, arise from the subbasal nerves (SBNs), & project apically. Quantitation of beta III tubulin & GAP43 within apical & basal layers of the corneal epithelium reveals that WT corneas have significantly more INTs in both basal & apical cell layers compared with SDC1-null corneas. Although SDC1-null corneas have fewer INTs, they are equally capable of extending toward the apical most cell layers. (B) In the 0.5-μm cross-sectional slices through the tissue, INTs appear discontinuous. In addition to beta III tubulin & GAP43, LAMP1 is shown in blue. LAMP1 is a lysosomal marker; co-localization of GAP43 and/or beta III tubulin within corneal epithelial cell lysosomes indicate that axon fragments have been phagocytozed & are being degraded (arrows). The sites indicated by the asterisks have been digitally enlarged 3-fold. LAMP1 was quantified in WT & SDC1-null corneas. Data show that cells within the apical layers express more LAMP1 compared with basal cells in both genotypes of mice. In addition, WT corneas express 2- to 3-fold more LAMP1 in apical & basal layers compared with SDC1-null corneas. Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28973369), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Eq, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GAP-43 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GAP-43 Antibody - BSA Free (NB300-143) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. Anti-GAP-43 Antibody: Cited in 102 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH.
• Localization: Neuronal Marker, developing and regenerating axons and growth cone.
• Marker: Neuronal Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GAP43
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Frozen 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1:10000
• Application Notes: This GAP43 antibody is useful for Immunocytochemistry/Immunofluorescence, and Western blot, where it recognizes a band at 43 kDa. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 29950987 and 27007292 respectively). Use in FLOW reported in scientific literature (PMID: 21614130).
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reactivity to Canine, Chicken, and Primate reported in scientific literature (PMID: 30647968, 30819546, and 24249398 respectively). Drosophila reactivity reported in scientific literature (PMID: 30819546).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAP-43 Antibody - BSA Free

• Axonal membrane protein GAP-43
• B-50
• Basp2
• calmodulin-binding protein P-57
• GAP43
• GAP-43
• growth associated protein 43
• Growth-associated protein 43
• nerve growth-related peptide GAP43
• Neural phosphoprotein B-50
• Neuromodulin
• neuron growth-associated protein 43
• PP46
• protein F1

Background

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAP-43 Antibody (NB300-143)(111)

Publications using NB300-143

• Shayya H The unfolded protein response couples neuronal identity to circuit formation in the developing mouse olfactory system Thesis 2023-01-01 (IF/IHC, Mouse)
• Acton L Investigating Mechanisms Responsible for the Pro-Regenerative Effects of Conditioning Electrical Stimulation Thesis 2022-01-01 (IHC-Fr, Mouse)
  
  Details:
  Adult 129S6/SvEvTac (wildtype) mice, IHC-Fr dilution used 1:500
• Brechbühl J, Lopes A, Wood D et al. Environmental stress promotes the persistence of juvenile traits in olfactory neurons as a protective mechanism iScience 2025-08-15 [PMID: 40948553]
• Sakthivelu, V;Schmitt, A;Odenthal, F;Ndoci, K;Touet, M;Shaib, AH;Chihab, A;Wani, GA;Nieper, P;Hartmann, GG;Pintelon, I;Kisis, I;Boecker, M;Eckert, NM;Iannicelli Caiaffa, M;Ibruli, O;Weber, J;Maresch, R;Bebber, CM;Chitsaz, A;Lütz, A;Kim Alves Carpinteiro, M;Morris, KM;Franchino, CA;Benz, J;Pérez-Revuelta, L;Soriano-Campos, JA;Huetzen, MA;Goergens, J;Jevtic, M;Jahn-Kelleter, HM;Zempel, H;Placzek, A;Hennrich, AA;Conzelmann, KK;Tumbrink, HL;Hunold, P;Isensee, J;Werr, L;Gaedke, F;Schauss, A;Minère, M;Müller, M;Fenselau, H;Liu, Y;Heimsoeth, A;Gülcüler Balta, GS;Walczak, H;Frezza, C;Jachimowicz, RD;George, J;Schmiel, M;Brägelmann, J;Hucho, T;von Karstedt, S;Peifer, M;Annibaldi, A;Hänsel-Hertsch, R;Persigehl, T;Grüll, H;Sos, ML;Reifenberger, G;Fischer, M;Adriaensen, D;Büttner, R;Sage, J;Brouns, I;Rad, R;Thomas, RK;Anstötz, M;Rizzoli, SO;Bergami, M;Motori, E;Reinhardt, HC;Beleggia, F; Functional synapses between neurons and small cell lung cancer Nature 2025-09-10 [PMID: 40931078]
• Belfiore L, Balakrishnan A, Touahri Y et al. Combinatorial expression of glial transcription factors induces Schwann cell-specific gene expression in mouse embryonic fibroblasts. Developmental dynamics : an official publication of the American Association of Anatomists 2025-06-19 [PMID: 40538023]
• Balakrishnan A Glial cells in health and disease Thesis 2020-01-01 (ICC/IF, Mouse)
• Hanell M Optimization of protocol for immunofluorescence stain to observe nerve infiltration and regeneration in cancer tissue Thesis 2022-01-01
• Zhang Y Morphogenetic roles of Epb41l5 and cell heterogeneity in early kidney development Thesis 2022-01-01 (IHC-P, Mouse)
  
  Details:
  Dilution used for IHC 1:200
• Hara Y, Jha MK, Huang JY et al. The IL-4-IL-4R alpha axis modulates olfactory neuroimmune signaling to induce loss of smell. Allergy 2024-10-17 [PMID: 39418114]
• Cavarischia-Rega C, Sharma K, Fitzgerald JC, Macek B. Proteome Dynamics in iPSC-Derived Human Dopaminergic Neurons Molecular & Cellular Proteomics : MCP 2024-09-07 [PMID: 39251023]
• Magdalena Blaszkiewicz, Tianyi Tao, Kofi Mensah-Arhin, Jake W Willows, Rhiannon Bates, Wei Huang, Lei Cao, Rosemary L Smith, Kristy L Townsend Gene therapy approaches for obesity-induced adipose neuropathy: Device-targeted AAV-mediated neurotrophic factor delivery to adipocytes in subcutaneous adipose. Molecular therapy : the journal of the American Society of Gene Therapy 2024-03-01 [PMID: 38429927]
• Huang SWJ Neurogenesis in the Olfactory System: The Functional Activity of Immature Olfactory Sensory Neurons and Postnatal-Born Granule Cells Thesis 2022-01-01 (IHC-Fr, Mouse)
• Merle-Nguyen L, Ando-Grard O, Bourgon C et Al. Early corticosteroid treatment enhances recovery from SARS-CoV-2 induced loss of smell in hamster Brain Behav Immun 2024-04-10 [PMID: 38367845]
• Mulc D, Smilovi? D, Krsnik Ž, Junakovi?-Munjas A et Al. Fetal development of the human amygdala J Comp Neurol 2024-01-30 [PMID: 38289194]
• Matthew D. Cain, N. Rubin Klein, Xiaoping Jiang, Hamid Salimi, Qingping Wu, Mark J. Miller, William B. Klimstra, Robyn S. Klein Post-exposure intranasal IFN alpha suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons Journal of Neuroinflammation 2024-01-17 [PMID: 38233868]
• Cain MD, Klein NR, Jiang X, Klein RS. Post-exposure intranasal IFN? suppresses replication and neuroinvasion of Venezuelan Equine Encephalitis virus within olfactory sensory neurons bioRxiv 2023-07-07 [PMID: 37425867] (Immunocytochemistry/ Immunofluorescence, Rat)
• Ueha R, Ito T, Ueha S et al. Evidence for the spread of SARS-CoV-2 and olfactory cell lineage impairment in close-contact infection Syrian hamster models Frontiers in Cellular and Infection Microbiology 2022-10-21 [PMID: 36339331]
• Tamaru T, Kobayakawa K, Saiwai H et al. Glial scar survives until the chronic phase by recruiting scar-forming astrocytes after spinal cord injury Experimental neurology 2023-01-01 [PMID: 36336030] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr 1:500
• Huang JS, Kunkhyen T, Rangel AN et al. Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb Nature communications 2022-10-19 [PMID: 36261441] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC 1:1000
• Efraim Y, Chen FYT, Cheong KN et al. A synthetic tear protein resolves dry eye through promoting corneal nerve regeneration Cell reports 2022-08-30 [PMID: 36044852] (IF/IHC, Mouse)
  
  Details:
  Dilutions: 1:400
• Singh HP, Shayler DWH, Fernandez GE et Al. An immature, dedifferentiated, and lineage-deconstrained cone precursor origin of N-Myc-initiated retinoblastoma Proc Natl Acad Sci U S A 2022-07-08 [PMID: 35867756]
• Olguin SL, Patel P, Buchanan CN et al. KHSRP loss increases neuronal growth and synaptic transmission and alters memory consolidation through RNA stabilization Communications biology 2022-07-07 [PMID: 35798971] (Immunocytochemistry/ Immunofluorescence, Rat)
• Ueha R, Ito T, Furukawa R et al. Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study Frontiers in Cellular and Infection Microbiology 2022-06-13 [PMID: 35770069]
• Mohanta SK, Peng L, Li Y et al. Neuroimmune cardiovascular interfaces control atherosclerosis Nature [PMID: 35477759] (IHC-P, Mouse)
• Ching K, Wang JT, Stearns T Long-range migration of centrioles to the apical surface of the olfactory epithelium eLife 2022-04-14 [PMID: 35420544] (ICC/IF, Mouse)
• Mazuelas H, MagallOn-Lorenz M, FernAndez-RodrIguez J et al. Modeling iPSC-derived human neurofibroma-like tumors in mice uncovers the heterogeneity of Schwann cells within plexiform neurofibromas Cell reports 2022-02-15 [PMID: 35172160] (Mouse)
• Pototskiy E, Vinokuroff K, Ojeda A Et al. Downregulation of CD40L-CD40 attenuates seizure susceptibility and severity of seizures Scientific reports 2021-08-26 [PMID: 34446808] (IF/IHC, Mouse)
• Mori E, Ueha R, Kondo K et al. Squamous and Respiratory Metaplasia After Olfactory Mucosal Resection Frontiers in Neuroscience 2021-07-20 [PMID: 34354563] (Immunohistochemistry)
• Paramos-De-Carvalho D, Martins I, Cristovao Am Et Al. Targeting senescent cells improves functional recovery after spinal cord injury Cell reports 2021-07-06 [PMID: 34233184]
• Li F, Lo TY, Miles L et al. The Atr-Chek1 pathway inhibits axon regeneration in response to Piezo-dependent mechanosensation Nature Communications 2021-06-22 [PMID: 34158506] (Immunocytochemistry/ Immunofluorescence, Rat)
• Zhu YT, Zhang Q, Xie HY et al. Environmental enrichment combined with fasudil promotes motor function recovery and axonal regeneration after stroke Neural regeneration research 2021-12-01 [PMID: 33907042] (IF/IHC, Mouse)
• Deng WW, Wu GY, Min LX et al. Optogenetic Neuronal Stimulation Promotes Functional Recovery After Spinal Cord Injury Frontiers in neuroscience 2021-04-09 [PMID: 33897353] (WB, Rat)
• Cao W, Zhang C, Chen R et al. A Novel Cerebroprotein Hydrolysate, CH1, Ameliorates Chronic Focal Cerebral Ischemia Injury by Promoting White Matter Integrity via the Shh/Ptch-1/Gli-1 Signaling Pathway Neuropsychiatric disease and treatment 2020-12-23 [PMID: 33380798] (WB, Mouse)
• Francis Rousset, Vivianne B. C. Kokje, Rebecca Sipione, Dominik Schmidbauer, German Nacher-Soler, Sten Ilmjärv, Marta Coelho, Stefan Fink, François Voruz, Antoun El Chemaly, Antoine Marteyn, Hubert Löwenheim, Karl-Heinz Krause, Marcus Müller, Rudolf Glückert, Pascal Senn Intrinsically Self-renewing Neuroprogenitors From the A/J Mouse Spiral Ganglion as Virtually Unlimited Source of Mature Auditory Neurons Frontiers in Cellular Neuroscience 2020-12-09 [PMID: 33362466]
• Zhang C, Cui L, He W et al. Dl-3-n-butylphthalide promotes neurite outgrowth of primary cortical neurons by Sonic Hedgehog signaling via upregulating Gap43 Experimental cell research 2020-12-06 [PMID: 33296663] (ICC/IF, Mouse)
• Niu J, Sanders SS, Jeong HK et al. Coupled Control of Distal Axon Integrity and Somal Responses to Axonal Damage by the Palmitoyl Acyltransferase ZDHHC17 Cell Rep 2020-11-17 [PMID: 33207199]
• Sanders SS, Hernandez LM, Soh H et al. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment eLife 2020-11-13 [PMID: 33185190] (Immunocytochemistry/ Immunofluorescence, Rat)
• Collura KM, Niu J, Sanders SS et al. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway J. Biol. Chem. 2020-09-21 [PMID: 32958558] (WB, Rat)
• Pal-Ghosh S, Tadvalkar G, Karpinski BA, Stepp MA Diurnal Control of Sensory Axon Growth and Shedding in the Mouse Cornea Invest. Ophthalmol. Vis. Sci. 2020-09-01 [PMID: 32870244] (ICC/IF, Mouse)
• Liu Y, Hall B, Lwin N et Al. Tissue Responses and Wound Healing following Laser Scleral Microporation for Presbyopia Therapy Transl Vis Sci Technol 2020-08-21 [PMID: 32818094]
• Lemons K, Fu Z, Ogura T, Lin W TRPM5-expressing Microvillous Cells Regulate Region-specific Cell Proliferation and Apoptosis During Chemical Exposure Neuroscience 2020-03-26 [PMID: 32224228]
• Hasegawa-Ishii S, Imamura F, Nagayama S et Al. Differential effects of nasal inflammation and odor deprivation on layer-specific degeneration of the mouse olfactory bulb eNeuro 2020-03-20 [PMID: 32220858] (Mouse)
• Merino P, Diaz A, Torre ER, Yepes M Urokinase-type plasminogen activator (uPA) regulates the expression and function of growth-associated protein 43 (GAP-43) in the synapse J. Biol. Chem. 2020-01-10 [PMID: 31819012] (ICC/IF, WB, Mouse)
• Bandeira, F, Yam, G H Et al. Three-Dimensional Neurite Characterization of Small Incision Lenticule Extraction Derived Lenticules. Invest Ophthalmol Vis Sci 2019-10-01 [PMID: 31639827] (ICC/IF, Drosophila melanogaster)
  
  Details:
  Citation using the Janiela Fluor 549 format of this antibody.
• Blaszkiewicz M, Willows JW, Dubois AL et al. Neuropathy and neural plasticity in the subcutaneous white adipose depot PLoS ONE 2019-09-11 [PMID: 31509546] (WB, Mouse)
• Azmi S, Jeziorska M, Ferdousi M et al. Early nerve fibre regeneration in individuals with type 1 diabetes after simultaneous pancreas and kidney transplantation Diabetologia 2019-06-07 [PMID: 31175373] (IF/IHC, Human)
• Zhou Y, Miles JR, Tavori H et al. Callosal septa express guidance cues and are paramedian guideposts for human corpus callosum development J. Anat. 2019-05-09 [PMID: 31070791] (WB)
• Sanders, SS;De Simone, FI;Thomas, GM; mTORC1 Signaling Is Palmitoylation-Dependent in Hippocampal Neurons and Non-neuronal Cells and Involves Dynamic Palmitoylation of LAMTOR1 and mTOR Front Cell Neurosci 2019-04-02 [PMID: 31001086] (WB, Human)
• Song Y, Li D, Farrelly O et al. The Mechanosensitive Ion Channel Piezo Inhibits Axon Regeneration Neuron 2019-02-15 [PMID: 30819546] (IF/IHC, Drosophila)
• Meritxell Carrió, Helena Mazuelas, Yvonne Richaud-Patin, Bernat Gel, Ernest Terribas, Imma Rosas, Senda Jimenez-Delgado, Josep Biayna, Leen Vendredy, Ignacio Blanco, Elisabeth Castellanos, Conxi Lázaro, Ángel Raya, Eduard Serra Reprogramming Captures the Genetic and Tumorigenic Properties of Neurofibromatosis Type 1 Plexiform Neurofibromas Stem Cell Reports 2019-01-31 [PMID: 30713041]
• Wang W, Jiang Z, Lu R et al. Effects of Renal Denervation via Renal Artery Adventitial Cryoablation on Atrial Fibrillation and Cardiac Neural Remodeling Cardiology Research and Practice 2018-12-11 [PMID: 30647968] (IHC-P, Canine)
• Ueha R, Kondo K, Ueha S, Yamasoba T. Dose-Dependent Effects of Insulin-Like Growth Factor 1 in the Aged Olfactory Epithelium Front Aging Neurosci 2018-11-20 [PMID: 30515092] (IF/IHC, Mouse)
• Wu Y, Ma L, Duyck K et al. A Population of Navigator Neurons Is Essential for Olfactory Map Formation during the Critical Period Neuron 2018-10-01 [PMID: 30482691]
• Renziehausen A, Wang H, Rao B et al. The renin angiotensin system (RAS) mediates bifunctional growth regulation in melanoma and is a novel target for therapeutic intervention Oncogene 2018-11-26 [PMID: 30478450] (WB, Human)
• Perry RBT, Hezroni H, Goldrich MJ, Ulitsky I. Regulation of Neuroregeneration by Long Noncoding RNAs. Molecular cell. [PMID: 30401432] (ICC/IF, Mouse)
• Zhang XM, Huang Y, Cong X et al. Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term. J. Cell. Physiol. 2018-11-01 [PMID: 30387129] (IF/IHC, Porcine)
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  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
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  Details:
  GAP43 antibody was used for IHC-P analysis of camel fetuses of several developmental stages and the staining was performed on vomeronasal organ (VNO) and the olfactory epithelium (OE) tissues. This antibody alongwith two others failed to detect camel's GAP45 in IHC-P assay which is correct in accordance with this antibody's immunogen sequence homology. NB300-143 is generated against AA216-226 of Rat/Mouse's Neuromodulin / Gap43 which does not show any sequence homology with camel's Neuromodulin.
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Review for GAP-43 Antibody (NB300-143) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Bryan Tinsley IHC-P Mouse 11/07/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: E15.5 Mouse
• Species: Mouse
• Lot: K-1

Bioinformatics

• Gene Symbol: GAP43