GAP-43 Antibody (5E8) - BSA Free

Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, NBP2-50052, dilution 1:1,000, in red, and costained with chicken pAb to GFAP, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, and GFAP antibody stains astroglia.

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Mixed rat neuronal cultures stained with NBP2-50052 (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 5E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GAP-43 Antibody (5E8) - BSA Free Summary

• Description: Novus Biologicals Mouse GAP-43 Antibody (5E8) - BSA Free (NBP2-50052) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GAP-43 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant full length human GAP-43. [UniProt# P17677]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAP43
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GAP-43 Antibody (5E8) - BSA Free

• Axonal membrane protein GAP-43
• B-50
• Basp2
• calmodulin-binding protein P-57
• GAP43
• GAP-43
• growth associated protein 43
• Growth-associated protein 43
• nerve growth-related peptide GAP43
• Neural phosphoprotein B-50
• Neuromodulin
• neuron growth-associated protein 43
• PP46
• protein F1

Background

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAP-43 Antibody (NBP2-50052)(3)

Publications using NBP2-50052

• Hanuscheck N, Thalman C, Domingues M et al. Interleukin-4 receptor signaling modulates neuronal network activity The Journal of experimental medicine 2022-06-06 [PMID: 35587822] (WB, Mouse)
• Zhang C, Cui L, He W et al. Dl-3-n-butylphthalide promotes neurite outgrowth of primary cortical neurons by Sonic Hedgehog signaling via upregulating Gap43 Experimental cell research 2020-12-06 [PMID: 33296663] (ICC/IF, WB, Mouse)
• Demyanenko, S, Gantsgorn, E Et al. Localization and Expression of Sirtuins 1, 2, 6 and Plasticity-Related Proteins in the Recovery Period after a Photothrombotic Stroke in Mice. J Stroke Cerebrovasc Dis 2020-10-01 [PMID: 32912518] (ICC/IF, Zebrafish)

Bioinformatics

• Gene Symbol: GAP43
• Uniprot:
  • P17677(Human)