Gankyrin Antibody

Images

 
Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.
Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NB100-1834] - Gankryrin antibody (1:500) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Flow (Intracellular): Gankyrin Antibody [NB100-1834] - Intracellular staining of Jurkat cells (1 x 10^6 cells/ml) with Gankyrin antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ZeSpecies Glossary
Applications WB, Flow-IC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.97 mg/ml

Gankyrin Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human Gankyrin protein sequence (between residues 100-200). [UniProt# O75832]
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500
  • Flow (Intracellular) 1:500
Application Notes
This Gankyrin antibody is useful in Western blot, where a band is observed ~24 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Gankyrin Lysate (NBL1-14895)

Reactivity Notes

Human. Predicted to react with mouse, rat, porcine, bovine, and zebrafish based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.97 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Gankyrin Antibody

  • 26S proteasome non-ATPase regulatory subunit 10
  • 26S proteasome regulatory subunit p28
  • ankyrin repeat protein
  • dJ889N15.2
  • gankyrin
  • hepatocellular carcinoma-associated protein p28-II
  • p28
  • p28(GANK)
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Background

Gankyrin is an ankyrin-repeat liver oncoprotein, that plays an important role in controlling the functions of the tumour suppressors RB and p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Gankyrin Antibody (NB100-1834): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Gankyrin Antibody (NB100-1834) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1834AF405
Alexa Fluor 488 Labeled NB100-1834AF488
Alexa Fluor 647 Labeled NB100-1834AF647
Alexa Fluor 700 Labeled NB100-1834AF700
Biotin Labeled NB100-1834B
DyLight 405 Labeled NB100-1834V
DyLight 488 Labeled NB100-1834G
DyLight 550 Labeled NB100-1834R
DyLight 650 Labeled NB100-1834C
FITC Labeled NB100-1834F
HRP Labeled NB100-1834H

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Bioinformatics Tool for Gankyrin Antibody (NB100-1834)

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Bioinformatics

Gene Symbol PSMD10