Gankyrin Antibodies
We offer Gankyrin Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each Gankyrin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Gankyrin Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Gankyrin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Gankyrin polyclonal antibodies and browse our Gankyrin monoclonal antibody catalog.
Alternate Names for Gankyrin Antibodies
anti-Gankyrin antibody, anti-PSMD10 antibody, anti-26S proteasome non-ATPase regulatory subunit 10 antibody, anti-26S proteasome regulatory subunit p28 antibody, anti-ankyrin repeat protein antibody, anti-dJ889N15.2 antibody, anti-hepatocellular carcinoma-associated protein p28-II antibody, anti-p28 antibody, anti-p28(GANK) antibody, anti-proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 antibody
9 Results for "gankyrin" in Primary Antibodies
![Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Western-Blot-NBP2-02199-img0015.jpg "Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.")
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Ovary tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0013.jpg)
![Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Jurkat cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Flow-Cytometry-NBP2-02199-img0002.jpg)
![Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Western-Blot-NBP2-02199-img0003.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0005.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded endometrium tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0011.jpg)
![Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Hela cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Flow-Cytometry-NBP2-02199-img0001.jpg)
![Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Western-Blot-NB100-1834-img0003.jpg "Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.")
![Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NB100-1834] - Gankryrin antibody (1:500) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1834-img0006.jpg)
![Flow (Intracellular): Gankyrin Antibody [NB100-1834] - Intracellular staining of Jurkat cells (1 x 10^6 cells/ml) with Gankyrin antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Flow-Intracellular-NB100-1834-img0009.jpg)
![Western Blot: Gankyrin Antibody (1F4) [NBP2-59400] - Lane 1: A549 cell lysates, Lane 2: PC3 cell lysates, Lane 3: K562 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: HeLa cell lysates, Lane 6: Jurkat cell lysates](http://images.novusbio.com/fullsize/Gankyrin-Antibody-1F4-Western-Blot-NBP2-59400-img0001.jpg "Western Blot: Gankyrin Antibody (1F4) [NBP2-59400] - Lane 1: A549 cell lysates, Lane 2: PC3 cell lysates, Lane 3: K562 cell lysates, Lane 4: HepG2 cell lysates, Lane 5: HeLa cell lysates, Lane 6: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: Gankyrin Antibody (1F4) [NBP2-59400] - Analysis of PSMD10 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD10 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Gankyrin-Antibody-1F4-Immunocytochemistry-Immunofluorescence-NBP2-59400-img0002.jpg)
![Western Blot: Gankyrin Antibody (C5) [NBP3-42158] - Sample: Lane1: Porcine Testis lysate; Lane2: A549 cell lysate; Lane3: HepG2 cell lysate; Lane4: K562 cell lysate; Lane5: PC3 cell lysate Primary Ab: 0.5ug/ml NBP3-42158; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42158_mouse-gankyrin-mab-c5-612202421243269.jpg "Western Blot: Gankyrin Antibody (C5) [NBP3-42158] - Sample: Lane1: Porcine Testis lysate; Lane2: A549 cell lysate; Lane3: HepG2 cell lysate; Lane4: K562 cell lysate; Lane5: PC3 cell lysate Primary Ab: 0.5ug/ml NBP3-42158; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Gankyrin Antibody (C2) [NBP3-42131] - Sample: Lane1: Porcine Testis lysate; Lane2: A549 cell lysate;](http://images.novusbio.com/fullsize/nbp3-42131_mouse-gankyrin-mab-c2-612202421351243.jpg "Western Blot: Gankyrin Antibody (C2) [NBP3-42131] - Sample: Lane1: Porcine Testis lysate; Lane2: A549 cell lysate;")
![Western Blot: Gankyrin Antibody [NBP1-82443] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Western-Blot-NBP1-82443-img0009.jpg "Western Blot: Gankyrin Antibody [NBP1-82443] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Gankyrin expression in invasive TGCC. a-d Gankyrin (red), POU5F1 (green; GCNIS cells), and MAGE-A4 (blue; spermatogonia) expression in GCNIS containing tubules from patients with invasive TGCC, yellow arrows - GCNIS cells, orange arrows - Sertoli cells. e and f Gankyrin (red), POU5F1 (green; seminoma cells), and MAGE-A4 (blue) expression in seminoma cells (e) and a mixed TGCC (e), inset on D - no primary antibody control. Scalebars - 50 um. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-019-6340-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82443-img0012.jpg)
![Immunohistochemistry-Paraffin: Gankyrin Antibody [NBP1-82443] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells of seminiferous ducts.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Immunohistochemistry-Paraffin-NBP1-82443-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Gankyrin Antibody [NBP1-82443] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-PSMD10 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Western-Blot-NBP1-82443-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Staining of human cell line A-431 shows positivity in cytoplasm.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82443-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Gankyrin Antibody [NBP1-82443] - Effects of Gankyrin knock-down on Gankyrin and POU5F1 expression. Representative Western blot for Gankyrin and POU5F1 expression in (V) vehicle and Gankyrin siRNA (T) transfected NTera2 cells. Tubulin was used as a loading control. Data analysed by paired t-test +/- SEM, ***p < 0.001, **p < 0.01. Each data point represents the mean of an individualexperiment, each with 3 replicates. Paired samples from an individual experiment are represented by the same colour. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/s12885-019-6340-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Knockdown-Validated-NBP1-82443-img0013.jpg)
![Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82443_rabbit-polyclonal-gankyrin-antibody-310202415363742.jpg)
![Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Gankyrin expression pre-invasive germ cells. a) 1 year old - maturation delay; b) 2 year old – maturation delay; c) 7 year old pre-invasive TGCC; d)17 year old pre-invasive TGCC; & e) 23 year old pre-invasive TGCC. Yellow arrows – GCNIS cells, orange arrows – Sertoli cells. This experiment was performed along with human fetal testis samples, no primary antibody control is the same as on Fig. 1, Scalebars - 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82443_rabbit-polyclonal-gankyrin-antibody-310202415371969.jpg)
![Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82443_rabbit-polyclonal-gankyrin-antibody-310202415371928.jpg)
![Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82443_rabbit-polyclonal-gankyrin-antibody-31020241517135.jpg)
![Western Blot: Gankyrin Antibody [NBP1-82443] - Effects of Gankyrin knock-down on Gankyrin & POU5F1 expression. Relative Gankyrin mRNA(a) & protein (b) expression in NTera2 cells after Gankyrin knock-down. Relative POU5F1 mRNA (c) & protein (d) expression in NTera2 cells after Gankyrin knock-down. e Representative Western blot for Gankyrin & POU5F1 expression in (V) vehicle & Gankyrin siRNA (T) transfected NTera2 cells. Tubulin was used as a loading control. Data analysed by paired t-test ± SEM, ***p < 0.001, **p < 0.01. Each data point represents the mean of an individualexperiment, each with 3 replicates. Paired samples from an individual experiment are represented by the same colour. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82443_rabbit-polyclonal-gankyrin-antibody-310202415284529.jpg)
![Immunocytochemistry/ Immunofluorescence: Gankyrin Antibody [NBP1-82443] - Representative images for Gankyrin expression in human fetal testis & normal adult testis. Gankyrin (red), POU5F1 (green; gonocyte) & MAGE-A4 (blue; prespermatogonia) expression in A), 11 week & B) 18 week old human fetal testis, counterstained with DAPI, yellow arrows – gonocytes, white arrows – pre-spermatogonia, green arrows – differentiating gonocyte, orange arrows – Sertoli cells, Scalebars – 50 μm. C) Representative image of Gankyrin expression in normal human adult testis immunostained for SOX9 (cyan; Sertoli cells), POU5F1 (green), MAGE-A4 (blue; spermatogonia) & Gankyrin (red), Cyan arrow – spermatogonia. Scalebars – 50 μm, insets – no primary antibody controls. w-weeks. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31744479), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-82443_rabbit-polyclonal-gankyrin-antibody-310202415334925.jpg)
![Gankyrin Antibody (OTI3F6) [Alexa Fluor® 350] [NBP2-71267AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.](http://images.novusbio.com/fullsize/nbp2-71267af350_mouse-gankyrin-mab-oti3f6-alexa-fluor-350-288202315203232.jpg "Gankyrin Antibody (OTI3F6) [Alexa Fluor® 350] [NBP2-71267AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.")