gamma Sarcoglycan Antibody

Images

 
Western Blot: gamma Sarcoglycan Antibody [NBP1-59744] - Lane 1 mouse Heart; 2 mouse Liver (expected negative); 3 mouse Liver; 4 mouse Quadriceps; 5 mouse Triceps; 6 mouse Triceps; 7 mouse Triceps. All lanes 25ug mouse ...read more
Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-59744] - Mouse tibialis anterior frozen sections. Image from verified customer review.
Western Blot: gamma Sarcoglycan Antibody [NBP1-59744] - Human Heart lysate, concentration 1.25ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

gamma Sarcoglycan Antibody Summary

Immunogen
Synthetic peptides corresponding to SGCG(sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)) The peptide sequence was selected from the middle region of SGCG. Peptide sequence FTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRS.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SGCG
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against SGCG and was validated on Western blot. Frozen section data from customer review.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-59744 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for gamma Sarcoglycan Antibody

  • 35 kDa dystrophin-associated glycoprotein
  • 35DAG
  • A4
  • DAGA4,35kD dystrophin-associated glycoprotein
  • DMDA
  • DMDA1
  • gamma sarcoglycan
  • gamma-sarcoglycan
  • gamma-SG
  • MAM
  • MGC130048
  • sarcoglycan, gamma (35kD dystrophin-associated glycoprotein)
  • sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
  • SCARMD2
  • SCG3LGMD2C
  • TYPE

Background

Gamma-sarcoglycan is one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin, probably to provide a link between the membrane associated cytoskeleton and the extracellular matrix. Defects in the protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C).Gamma-sarcoglycan is one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin, probably to provide a link between the membrane associated cytoskeleton and the extracellular matrix. Defects in the protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for gamma Sarcoglycan Antibody (NBP1-59744) (0)

There are no publications for gamma Sarcoglycan Antibody (NBP1-59744).
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Reviews for gamma Sarcoglycan Antibody (NBP1-59744) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-59744:
Filter by Applications
IHC-Fr
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen gamma Sarcoglycan NBP1-59744
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 08/28/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse tibialis anterior
SpeciesMouse

Blocking

Blocking DetailsGoat Normal Serum, 15 min, room temperature

Primary Anitbody

Dilution Ratio1:50, 60min at 37C

Secondary Antibody

Secondary Descriptiongoat anti rabbit IgF-FITC
Secondary Manufacturer Cat#Sigma F9887
Secondary Concentration1:300

Details

Detection NotesImage at 20x, Olympus B-65 Microscope/Camera
Fixation DetailsCut section at 5um, adhered to charged slide. 1) PBS 3x for 5min each 2) 1% Triton for 20min 3)PBS 3x 5min each; all room temp
Wash DescriptionPBS 3x, 5min each.
Western Blot gamma Sarcoglycan NBP1-59744
Enlarge
reviewed by:
Anonymous
WB Mouse 08/13/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse Heart, Liver, Quadriceps, and Triceps
SpeciesMouse
LotQC17604-100331
CommentsIncreasing exposure time to ~24 hours did increase signal, but also increased background. Increasing the secondary antibody dilution may help lower background at high exposure times.

Blocking

Blocking Details5% Milk, TBS-Tween 0.1%, 1 hour 4C

Primary Anitbody

Dilution Ratio1:1000 in 5% Milk, TBS-Tween 0.1%. Incubated overnight (~14 hrs) at 4C

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit, HRP
Secondary Manufacturer Cat#Santa Cruz, sc-2305
Secondary Concentration1:10,000

Details

Detection NotesPierce ECL Western Blotting Substrat, # 32106. Exposure time 2 hours.

Comments

CommentsIncreasing exposure time to ~24 hours did increase signal, but also increased background. Increasing the secondary antibody dilution may help lower background at high exposure times.

Product General Protocols

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Video Protocols

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FAQs for gamma Sarcoglycan Antibody (NBP1-59744) (0)

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Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
08/28/2014
Application: IHC-Fr
Species: Mouse

Anonymous
08/13/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SGCG
Uniprot