Galectin-3BP/MAC-2BP/LGALS3BP Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP ...read more
Galectin‑3BP/MAC‑2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Galectin-3BP/MAC-2BP/LGALS3BP Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP
Val19-Asp585
Accession # Q08380
Specificity
Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Galectin‑3BP/MAC‑2BP is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS3BP
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 10 ug/mL
  • Immunocytochemistry 1-15 ug/mL
Control
Galectin-3BP/MAC-2BP/LGALS3BP Knockout 293T Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2226 in the following applications:

Publications
Read Publications using
AF2226 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-3BP/MAC-2BP/LGALS3BP Antibody [Unconjugated]

  • 90K
  • Basement membrane autoantigen p105
  • BTBD17B
  • galectin-3-binding protein
  • Galectin3BP
  • Galectin-3BP
  • L3 antigen
  • Lectin galactoside-binding soluble 3-binding protein
  • lectin, galactoside-binding, soluble, 3 binding protein
  • LGALS3BP
  • M2BP
  • Mac-2 BP
  • Mac-2-binding protein
  • MAC2BP
  • MAC-2BP
  • MAC-2-BP
  • serum protein 90K
  • Tumor-associated antigen 90K

Background

Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.

  1. Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
  2. Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
  3. Sasaki, T. et al. (1998) EMBO J. 17:1606.
  4. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
  5. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
  6. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
  7. Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
  8. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-3BP/MAC-2BP/LGALS3BP Antibody (AF2226)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(2)
Western Blot
(7)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using AF2226 Applications Species
G Dolgormaa, N Harimoto, N Ishii, T Yamanaka, K Hagiwara, M Tsukagoshi, T Igarashi, A Watanabe, N Kubo, K Araki, T Handa, T Yokobori, T Oyama, H Kuwano, K Shirabe Mac-2-binding protein glycan isomer enhances the aggressiveness of hepatocellular carcinoma by activating mTOR signaling Br. J. Cancer, 2020;0(0):. 2020 [PMID: 32624579] (IHC, Human) IHC Human
T Seki, M Kanagawa, K Kobayashi, H Kowa, N Yahata, K Maruyama, N Iwata, H Inoue, T Toda Galectin 3-binding protein suppresses amyloid-&beta production by modulating &beta-cleavage of amyloid precursor protein J. Biol. Chem., 2020;295(11):3678-3691. 2020 [PMID: 31996371] (Western Blot, Human) Western Blot Human
JK Woo, JE Jang, JH Kang, JK Seong, YS Yoon, HC Kim, SJ Lee, SH Oh Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line Mol. Cancer Ther, 2017;0(0):. 2017 [PMID: 28336809] (Western Blot, Human) Western Blot Human
SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques J. Clin. Invest, 2017;0(0):. 2017 [PMID: 28319050] (IHC, Human) IHC Human
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner Sci Rep, 2016;6(0):32736. 2016 [PMID: 27604950] (Western Blot, Human) Western Blot Human
Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6. J. Virol., 2012;86(12):6620-31. 2012 [PMID: 22496229] (Western Blot, Human) Western Blot Human
Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma. Cancer Res, 2012;72(9):2228-38. 2012 [PMID: 22389450] (Western Blot, Human) Western Blot Human
Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31. Exp. Cell Res., 2011;317(7):976-93. 2011 [PMID: 21182835] (Western Blot, Human) Western Blot Human
Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma. J. Biol. Chem., 2008;283(27):18573-81. 2008 [PMID: 18450743] (Western Blot, Human) Western Blot Human

Reviews for Galectin-3BP/MAC-2BP/LGALS3BP Antibody (AF2226) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2226:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22496229
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22496229

Details

Detection NotesSee PMID 22496229

Comments

Commentsnot for ipSee PMID 22496229
  4
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21182835
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21182835

Details

Detection NotesSee PMID 21182835

Comments

CommentsPublished: See PMID 21182835

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/06/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LGALS3BP
Uniprot