Galectin-3BP/MAC-2BP/LGALS3BP Antibody [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP
Accession # Q08380
Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Galectin‑3BP/MAC‑2BP is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Immunocytochemistry 1-15 ug/mL
- Simple Western 10 ug/mL
- Western Blot 0.2 ug/mL
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Galectin-3BP/MAC-2BP/LGALS3BP Antibody [Unconjugated]
- Basement membrane autoantigen p105
- galectin-3-binding protein
- L3 antigen
- Lectin galactoside-binding soluble 3-binding protein
- lectin, galactoside-binding, soluble, 3 binding protein
- Mac-2 BP
- Mac-2-binding protein
- serum protein 90K
- Tumor-associated antigen 90K
Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.
- Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
- Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
- Sasaki, T. et al. (1998) EMBO J. 17:1606.
- Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
- Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
- Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
- Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
- Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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