Galectin-14/LGALS14 Antibody (511124)

Western blot shows lysates of human eosinophil. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-14 Monoclonal Antibody (Catalog # MAB5744) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin-14 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 511124
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Galectin-14/LGALS14 Antibody (511124) Summary

• Immunogen: E. coli-derived recombinant human Galectin-14
  Met1-Asp139
  Accession # Q8TCE9
• Specificity: Detects human Galectin-14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, -10, or recombinant mouse Galectin-9 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGALS14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-14/LGALS14 Antibody (511124)

• Charcot-Leyden crystal protein 2
• CLC2
• Gal-14
• Galectin14
• Galectin-14
• lectin, galactoside-binding, soluble, 14
• LGALS14
• MGC22235
• Placental Protein 13-Like Protein
• PPL13

Background

Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-14/LGALS14 Antibody (MAB5744)(1)

Publication using MAB5744

• Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease Sci Rep, 2016-09-23;6(0):32382. 2016-09-23 [PMID: 27660926] (IHC, Human)

Bioinformatics

• Gene Symbol: LGALS14
• Uniprot:
  • Q8TCE9()