HepaCAM Antibody (419305) [Unconjugated]

Western blot shows lysates of human brain (motor cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human HepaCAM Monoclonal Antibody (Catalog # MAB4108) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HepaCAM at approximately 45-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 419305
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HepaCAM Antibody (419305) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HepaCAM
  Val34-Tyr242
  Accession # Q14CZ8
• Specificity: Detects human HepaCAM in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HEPACAM
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HepaCAM Antibody (419305) [Unconjugated]

• cancer susceptibility
• FLJ25530
• glial cell adhesion molecule
• GlialCAM
• HepaCAM
• hepatic and glial cell adhesion molecule
• hepatocyte cell adhesion molecule
• Protein hepaCAM

Background

HepaCAM (hepatocyte cell adhesion molecule) is a 50-75 kDa, type I transmembrane glycoprotein that belongs to the Ig-superfamily. It forms homodimers on the cell surface and promotes cell spreading and motility. Human HepaCAM is 382 aa in length. It contains a 206 aa extracellular domain (ECD) (aa 35‑240) and a 152 aa cytoplasmic region. The ECD has two C2 Ig-like domains. There is one potential truncated isoform that shows an alternate start site at Met200. Over aa 34‑242, human HepaCAM shares 99% and 98% aa sequence identity with mouse and dog HepaCAM, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HepaCAM Antibody (MAB4108)(7)

Publications using MAB4108

• MC Krawczyk, L Pan, AJ Zhang, Y Zhang Lymphocyte deficiency alters the transcriptomes of oligodendrocytes, but not astrocytes or microglia PLoS ONE, 2023-02-24;18(2):e0279736. 2023-02-24 [PMID: 36827449] (Immunoprecipitation, Mouse)
• J Sun, S Osenberg, A Irwin, LH Ma, N Lee, Y Xiang, F Li, YW Wan, IH Park, M Maletic-Sa, N Ballas Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation Cell Reports, 2023-01-05;42(1):111942. 2023-01-05 [PMID: 36640327] (IHC, Human)
• A Nolle, I van Dijken, CM Waelti, D Calini, J Bryois, E Lezan, S Golling, A Augustin, L Foo, JJM Hoozemans Enrichment of Glial Cells From Human Post-mortem Tissue for Transcriptome and Proteome Analysis Using Immunopanning Frontiers in Cellular Neuroscience, 2021-12-13;15(0):772011. 2021-12-13 [PMID: 34966261] (Immunopanning, Human)
• KA Guttenplan, MK Weigel, P Prakash, PR Wijewardha, P Hasel, U Rufen-Blan, AE Münch, JA Blum, J Fine, MC Neal, KD Bruce, AD Gitler, G Chopra, SA Liddelow, BA Barres Neurotoxic reactive astrocytes induce cell death via saturated lipids Nature, 2021-10-06;0(0):. 2021-10-06 [PMID: 34616039] (Bioassay, Mouse)
• KA Guttenplan, MK Weigel, DI Adler, J Couthouis, SA Liddelow, AD Gitler, BA Barres Knockout of reactive astrocyte activating factors slows disease progression in an ALS mouse model Nat Commun, 2020-07-27;11(1):3753. 2020-07-27 [PMID: 32719333] (Cell Culture, Mouse)
• D Henrik Hei, VM Ravi, SP Behringer, JH Frenking, J Wurm, K Joseph, NWC Garrelfs, J Strähle, S Heynckes, J Grauvogel, P Franco, I Mader, M Schneider, AL Potthoff, D Delev, UG Hofmann, C Fung, J Beck, R Sankowski, M Prinz, O Schnell Tumor-associated reactive astrocytes aid the evolution of immunosuppressive environment in glioblastoma Nat Commun, 2019-06-11;10(1):2541. 2019-06-11 [PMID: 31186414] (Flow Cytometry, IHC, Immunopanning, Human)
• HepaCAM associates with connexin 43 and enhances its localization in cellular junctions Sci Rep, 2016-11-07;6(0):36218. 2016-11-07 [PMID: 27819278] (Western Blot, Human)

Review for HepaCAM Antibody (MAB4108) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Pig.

reviewed by: Verified Customer ICC Pig 06/07/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: pig SC cells
• Species: Pig
• Lot: N/A

Bioinformatics

• Gene Symbol: HEPACAM
• Uniprot:
  • Q14CZ8()