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Galectin-1 Antibody

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Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin‑1 Antigen ...read more
Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, CyTOF-ready, ELISA(Cap), Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Galectin-1 Antibody Summary

Immunogen
E. coli-derived recombinant mouse Galectin-1
Ala2-Glu135
Accession # P16045
Specificity
Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin‑1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin‑8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse Galectin-1 Antibody (Catalog # AF1245)
ELISA Detection: Mouse Galectin-1 Biotinylated Antibody (Catalog # BAF1245)
Standard: Recombinant Mouse Galectin-1 (Catalog # 1245-GA)
Publications
Read Publications using
AF1245 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-1 Antibody

  • 14 kDa laminin-binding protein
  • 14 kDa lectin
  • Beta-galactoside-binding lectin L-14-I
  • BHL
  • DKFZp686E23103
  • GAL1
  • gal-1
  • Galaptin
  • galectin 1
  • Galectin1
  • Galectin-1
  • GBP
  • HBL
  • HLBP14
  • HPL
  • L-14
  • Lactose-binding lectin 1
  • Lectin galactoside-binding soluble 1
  • lectin, galactoside-binding, soluble, 1
  • LGALS1
  • Putative MAPK-activating protein PM12
  • S-Lac lectin 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).

  1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2001) Biochimie 83:667.
  4. R&D Systems’ Cytokine Bulletin, Summer (2002).
  5. Goldring, K. et al. (2002) J. Cell Science 115:355.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-1 Antibody (AF1245)(16)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 7 applications: ELISA Capture, Flow Cytometry, ICC, IHC, IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Capture
(1)
Flow Cytometry
(1)
ICC
(3)
IHC
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Western Blot
(8)
All Applications
Filter By Species
Mouse
(15)
Rat
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF1245 Applications Species
LG Morosi, AM Cutine, AJ Cagnoni, MN Manselle-C, DO Croci, JP Merlo, RM Morales, M May, JM Pérez-Sáez, MR Girotti, SP Méndez-Hue, B Pucci, AH Gil, SP Huernos, GH Docena, AM Sambuelli, MA Toscano, GA Rabinovich, KV Mariño Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits Science Advances, 2021;7(25):. 2021 [PMID: 34144987] (ELISA Capture, Mouse) ELISA Capture Mouse
V Baloche, J Rivière, TBT Tran, A Gelin, O Bawa, N Signolle, MBK Diop, P Dessen, S Beq, M David, P Busson Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model Scientific Reports, 2021;11(1):5227. 2021 [PMID: 33664349] (Western Blot, Mouse) Western Blot Mouse
JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche Stem Cell Res Ther, 2020;11(1):425. 2020 [PMID: 32993816] (Western Blot, Mouse) Western Blot Mouse
HL Law, RD Wright, AJ Iqbal, LV Norling, D Cooper A Pro-resolving Role for Galectin-1 in Acute Inflammation Front Pharmacol, 2020;11(0):274. 2020 [PMID: 32265698] (Flow Cytometry, Mouse) Flow Cytometry Mouse
MS Tsai, MT Chiang, DL Tsai, CW Yang, HS Hou, YR Li, PC Chang, HH Lin, HY Chen, IS Hwang, PK Wei, CP Hsu, KI Lin, FT Liu, LY Chau Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics Sci Rep, 2018;8(1):11497. 2018 [PMID: 30065303] (Western Blot, Mouse) Western Blot Mouse
N Yang, W Zhang, T He, Y Xing Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 2017;0(0):. 2017 [PMID: 28257831] (IHC, Mouse) IHC Mouse
Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, Jaiswal J Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair. Nat Commun, 2014;5(0):5646. 2014 [PMID: 25534348] (Western Blot, Mouse) Western Blot Mouse
Baker G, Chockley P, Yadav V, Doherty R, Ritt M, Sivaramakrishnan S, Castro M, Lowenstein P Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity. Cancer Res, 2014;74(18):5079-90. 2014 [PMID: 25038230] (Western Blot, Mouse) Western Blot Mouse
Szebeni G, Kriston-Pal E, Blazso P, Katona R, Novak J, Szabo E, Czibula A, Fajka-Boja R, Hegyi B, Uher F, Krenacs L, Joo G, Monostori E Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion. PLoS ONE, 2012;7(7):e41372. 2012 [PMID: 22844466] (ICC, Mouse) ICC Mouse
Impaired spatial and contextual memory formation in galectin-1 deficient mice. Mol Brain, 2011;4(0):33. 2011 [PMID: 21884595] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol LGALS1
Uniprot