Galectin-1 Antibody [Unconjugated]

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." title="Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." />

Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." > FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." title="Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." />

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # <a class=

Simple Western lane view shows lysates of L6 rat myoblast cell line, C2C12 mouse myoblast cell line, THP‑1 human acute monocytic leukemia cell line and Neuro‑2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 18 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

HAF017). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF017). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, L6 rat myoblast cell line, C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line and Mouse Thymus. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin‑1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line, L6 rat myoblast cell line, C2C12 mouse myoblast cell line, Neuro‑2A mouse neuroblastoma cell line and Mouse Thymus. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

1245-GA) was serially diluted and captured by Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 1245-GA) was serially diluted and captured by Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Mouse Galectin‑1 (Catalog # 1245-GA) was serially diluted and captured by Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Mouse Galectin‑1 (Catalog # <a class=

1245-GA) was serially diluted and captured by Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 1245-GA) was serially diluted and captured by Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Mouse Galectin‑1 (Catalog # 1245-GA) was serially diluted and captured by Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Mouse Galectin‑1 (Catalog # <a class=

• Reactivity: Mu
• Applications: WB, Simple Western, IHC, CyTOF-ready, ELISA(Cap), Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Galectin-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Galectin-1
  Ala2-Glu135
  Accession # P16045
• Specificity: Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin‑1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin‑8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LGALS1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Galectin-1 Antibody (Catalog # AF1245)
  ELISA Detection: Mouse Galectin-1 Biotinylated Antibody (Catalog # BAF1245)
  Standard: Recombinant Mouse Galectin-1 (Catalog # 1245-GA)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-1 Antibody [Unconjugated]

• 14 kDa laminin-binding protein
• 14 kDa lectin
• Beta-galactoside-binding lectin L-14-I
• BHL
• DKFZp686E23103
• GAL1
• gal-1
• Galaptin
• galectin 1
• Galectin1
• Galectin-1
• GBP
• HBL
• HLBP14
• HPL
• L-14
• Lactose-binding lectin 1
• Lectin galactoside-binding soluble 1
• lectin, galactoside-binding, soluble, 1
• LGALS1
• Putative MAPK-activating protein PM12
• S-Lac lectin 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).

Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5).

1. Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
3. Hughes, R.C. (2001) Biochimie 83:667.
4. R&D Systems’ Cytokine Bulletin, Summer (2002).
5. Goldring, K. et al. (2002) J. Cell Science 115:355.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-1 Antibody (AF1245)(22)

Publications using AF1245

• Julia Femel, Luuk van Hooren, Melanie Herre, Jessica Cedervall, Falk Saupe, Elisabeth J. M. Huijbers et al. Vaccination against galectin-1 promotes cytotoxic T-cell infiltration in melanoma and reduces tumor burden Cancer Immunology, Immunotherapy 2022-08-01 [PMID: 35018481]
• LG Morosi, AM Cutine, AJ Cagnoni, MN Manselle-C, DO Croci, JP Merlo, RM Morales, M May, JM Pérez-Sáez, MR Girotti, SP Méndez-Hue, B Pucci, AH Gil, SP Huernos, GH Docena, AM Sambuelli, MA Toscano, GA Rabinovich, KV Mariño Control of intestinal inflammation by glycosylation-dependent lectin-driven immunoregulatory circuits Science Advances, 2021-06-18;7(25):. 2021-06-18 [PMID: 34144987] (ELISA Capture, Mouse)
• V Baloche, J Rivière, TBT Tran, A Gelin, O Bawa, N Signolle, MBK Diop, P Dessen, S Beq, M David, P Busson Serial transplantation unmasks galectin-9 contribution to tumor immune escape in the MB49 murine model Scientific Reports, 2021-03-04;11(1):5227. 2021-03-04 [PMID: 33664349] (Western Blot, Mouse)
• Chiang MT, Chen IM, Hsu FF et al. Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation Arterioscler Thromb Vasc Biol 11/5/2020 [PMID: 33147994]
• JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche Stem Cell Res Ther, 2020-09-29;11(1):425. 2020-09-29 [PMID: 32993816] (Western Blot, Mouse)
• HL Law, RD Wright, AJ Iqbal, LV Norling, D Cooper A Pro-resolving Role for Galectin-1 in Acute Inflammation Front Pharmacol, 2020-03-20;11(0):274. 2020-03-20 [PMID: 32265698] (Flow Cytometry, Mouse)
• Dhanya K. Nambiar, Todd Aguilera, Hongbin Cao, Shirley Kwok, Christina Kong, Joshua Bloomstein et al. Galectin-1–driven T cell exclusion in the tumor endothelium promotes immunotherapy resistance Journal of Clinical Investigation 2019-11-11 [PMID: 31710313]
• Heidi L. Reesink, Alan J. Nixon, Jin Su, Sherry Liu, Ryan M. Sutton, Sabine Mann et al. Galectins-1 and-3 Increase in Equine Post-traumatic Osteoarthritis Frontiers in Veterinary Science 2018-11-20 [PMID: 30525048]
• MS Tsai, MT Chiang, DL Tsai, CW Yang, HS Hou, YR Li, PC Chang, HH Lin, HY Chen, IS Hwang, PK Wei, CP Hsu, KI Lin, FT Liu, LY Chau Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics Sci Rep, 2018-07-31;8(1):11497. 2018-07-31 [PMID: 30065303] (IHC, Western Blot, Mouse)
• Daniel Abebayehu, Andrew Spence, Barbara D. Boyan, Zvi Schwartz, John J. Ryan, Michael J. McClure Galectin-1 promotes an M2 macrophage response to polydioxanone scaffolds Journal of Biomedical Materials Research Part A 2017-09-01 [PMID: 28544348]
• N Yang, W Zhang, T He, Y Xing Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy Exp. Eye Res, 2017-02-28;0(0):. 2017-02-28 [PMID: 28257831] (IHC, Mouse)
• Andrew D. Gaudet, David R. Sweet, Nicole K. Polinski, Zhen Guan, Phillip G. Popovich Galectin-1 in injured rat spinal cord: Implications for macrophage phagocytosis and neural repair Molecular and Cellular Neuroscience 2015-01-01 [PMID: 25542813]
• Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, Jaiswal J Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair. Nat Commun, 2014-12-23;5(0):5646. 2014-12-23 [PMID: 25534348] (Western Blot, Mouse)
• Baker G, Chockley P, Yadav V, Doherty R, Ritt M, Sivaramakrishnan S, Castro M, Lowenstein P Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity. Cancer Res, 2014-07-18;74(18):5079-90. 2014-07-18 [PMID: 25038230] (ICC, Western Blot, Mouse, Rat)
• Szebeni G, Kriston-Pal E, Blazso P, Katona R, Novak J, Szabo E, Czibula A, Fajka-Boja R, Hegyi B, Uher F, Krenacs L, Joo G, Monostori E Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion. PLoS ONE, 2012-07-23;7(7):e41372. 2012-07-23 [PMID: 22844466] (ICC, Mouse)
• Impaired spatial and contextual memory formation in galectin-1 deficient mice. Mol Brain, 2011-09-01;4(0):33. 2011-09-01 [PMID: 21884595] (IHC-Fr, Mouse)
• Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect. Am. J. Pathol., 2011-03-01;178(3):1201-9. 2011-03-01 [PMID: 21356371] (IHC-P, Western Blot, Mouse)
• Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia. J. Neurochem., 2010-12-02;116(2):217-26. 2010-12-02 [PMID: 21054390] (Western Blot, Rat)
• Shimizu M, Khoshnoodi J, Akimoto Y, Kawakami H, Hirano H, Higashihara E, Hosoyamada M, Sekine Y, Kurayama R, Kurayama H, Joh K, Hirabayashi J, Kasai K, Tryggvason K, Ito N, Yan K Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome. Lab. Invest., 2008-12-15;89(2):178-95. 2008-12-15 [PMID: 19079321] (Western Blot, Mouse, Rat)
• Plachta N, Annaheim C, Bissiere S, Lin S, Ruegg M, Hoving S, Muller D, Poirier F, Bibel M, Barde YA Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells. Nat. Neurosci., 2007-05-07;10(6):712-9. 2007-05-07 [PMID: 17486104] (ICC, IHC-Fr, Neutralization, Mouse, Rat)
• Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection. Am. J. Pathol., 2007-02-01;170(2):546-56. 2007-02-01 [PMID: 17255323] (ICC, IHC, Mouse)
• Endharti AT, Zhou YW, Nakashima I, Suzuki H Galectin-1 supports survival of naive T cells without promoting cell proliferation. Eur. J. Immunol., 2005-01-01;35(1):86-97. 2005-01-01 [PMID: 15593120] (Immunoprecipitation, Mouse)

Bioinformatics

• Gene Symbol: LGALS1
• Uniprot:
  • P16045()