GALE Antibody (OTI1C4) - Azide and BSA Free

Western Blot: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GALE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALE.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Human lymph node tissue using anti-GALE mouse monoclonal antibody.

Western Blot: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GALE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GALE mouse monoclonal antibody.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GALE mouse monoclonal antibody.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GALE mouse monoclonal antibody.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GALE mouse monoclonal antibody.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Human Kidney tissue using anti-GALE mouse monoclonal antibody.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Human liver tissue using anti-GALE mouse monoclonal antibody.

Immunohistochemistry: GALE Antibody (OTI1C4) - Azide and BSA Free [NBP2-70793] - Staining of paraffin-embedded Human prostate tissue using anti-GALE mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, IHC
• Clone: OTI1C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GALE Antibody (OTI1C4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse GALE Antibody (OTI1C4) - Azide and BSA Free (NBP2-03390) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human GALE (NP_000394) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GALE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 38.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GALE Antibody (OTI1C4) - Azide and BSA Free

• EC 5.1.3
• EC 5.1.3.2
• FLJ95174
• FLJ97302
• Galactowaldenase
• short chain dehydrogenase/reductase family 1E, member 1
• UDP galactose-4'-epimerase
• UDP-
• UDP-galactose 4-epimerase
• UDP-galactose-4-epimerase
• UDP-glucose 4-epimerase

Background

GALE encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GALE