GALE Antibody (OTI1C4)

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Western Blot: GALE Antibody (1C4) [NBP2-03390] Analysis of extracts (35ug) from 9 different cell lines by using anti-GALE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: ...read more
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human prostate tissue using anti-GALE mouse monoclonal antibody.
Western Blot: GALE Antibody (1C4) [NBP2-03390] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GALE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GALE mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GALE mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GALE mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GALE mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human Kidney tissue using anti-GALE mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human liver tissue using anti-GALE mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GALE Antibody (1C4) [NBP2-03390] - Staining of paraffin-embedded Human lymph node tissue using anti-GALE mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, IHC
Clone
OTI1C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GALE Antibody (OTI1C4) Summary

Immunogen
Full length human recombinant protein of human GALE (NP_000394) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GALE
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
38.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GALE Antibody (OTI1C4)

  • EC 5.1.3
  • EC 5.1.3.2
  • FLJ95174
  • FLJ97302
  • Galactowaldenase
  • short chain dehydrogenase/reductase family 1E, member 1
  • UDP galactose-4'-epimerase
  • UDP-
  • UDP-galactose 4-epimerase
  • UDP-galactose-4-epimerase
  • UDP-glucose 4-epimerase

Background

This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GALE
Entrez