Western Blot: GADD153/CHOP Antibody [NBP2-13172] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in ...read more
Western Blot: GADD153/CHOP Antibody [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. Western blot assays showing the effects of psoralen and thapsigargin on ER-stress related ...read more
Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - Mouse liver tissue. Antigen retreival: Citrate buffer pH=6.0,Blocking: 10% BSA in 0.1%Tween in TBS, Primary antibody: 1:100 in 1% BSA in 0.1%Tween in ...read more
Flow Cytometry: GADD153/CHOP Antibody [NBP2-13172] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody NBP2-13172F (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Biological Strategies: Western Blot: GADD153/CHOP Antibody [NBP2-13172] - CHOP/GADD153 Antibody [NBP2-13172] - WB analysis of CHOP/GADD153 in tunicamycin treated (+) and untreated (-) HeLa cell lysate.
Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - IHC staining of CHOP/GADD153 in mouse colon using DAB with hematoxylin counterstain.
Human, mouse, rat, and primate. Mouse reactivity reported in scientific literature (PMID:32814096).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4)
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for GADD153/CHOP Antibody
C/EBP-homologous protein 10
CCAAT/enhancer-binding protein homologous protein
DNA damage-inducible transcript 3 protein
DNA-damage-inducible transcript 3
Growth arrest and DNA damage-inducible protein GADD153
GADD153 also known as DNA damage-inducible transcript 3 protein (DDIT3), C/EBP-homologous protein (CHOP), CHOP10, and CEBPZ, is a growth arrest and DNA damage inducible gene that encodes a C/EBP-related protein. GADD153 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. GADD153 can be found in the nucleus and its expression is induced by cellular stresses including nutrient deprivation, endoplasmic reticulum stress, and metabolic perturbations. The GADD153 protein functions as a dominant-negative inhibitor that inhibits DNA-binding activity with other C/EBP members, such as C/EBP and LAP (liver activator protein), by forming heterodimers. Fusion of GADD153 and FUS on chromosome 16 or EWSR1 on chromosome 22, induced by translocation, generates chimeric proteins in myxoid liposarcomas, also known as Ewing sarcoma. GADD153 is implicated in adipogenesis and promotes apoptosis in cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
CHOP: NBP2-13172 Antigen retreival: Citrate buffer pH=6.0 Blocking: 10% BSA in 0.1%Tween in TBS Primary antobidy: 1:100 in 1% BSA in 0.1%Tween in TBS Incubation: overnight at 4 degree 2ndry antibody: 1:200 incubation for 1 hour at room temperature AB complex: 1:100 for 1hour at room temprature Develpment. DAB
Product General Protocols
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