GADD153/CHOP Antibody

Images

 
Western Blot: GADD153/CHOP Antibody [NBP2-13172] - NASTRp induces ER stress and eventually leads to cell death with Bim up-regulation. NASTRp induced ER stress and activated UPR led to apoptosis with Bim induction in ...read more
Western Blot: GADD153/CHOP Antibody [NBP2-13172] - Effects of psoralen on ER-stress related protein expression in SMMC7721. Western blot assays showing the effects of psoralen and thapsigargin on ER-stress related ...read more
Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - Mouse liver tissue. Antigen retreival: Citrate buffer pH=6.0,Blocking: 10% BSA in 0.1%Tween in TBS, Primary antibody: 1:100 in 1% BSA in 0.1%Tween in ...read more
Flow Cytometry: GADD153/CHOP Antibody [NBP2-13172] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody NBP2-13172F (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Biological Strategies: Western Blot: GADD153/CHOP Antibody [NBP2-13172] - CHOP/GADD153 Antibody [NBP2-13172] - WB analysis of CHOP/GADD153 in tunicamycin treated (+) and untreated (-) HeLa cell lysate.
Immunohistochemistry: GADD153/CHOP Antibody [NBP2-13172] - IHC staining of CHOP/GADD153 in mouse colon using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.67 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

GADD153/CHOP Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human CHOP/GADD153 protein (between residues 100-150) [UniProt P35638]
Localization
Cytoplasm when unactivated, translocation to nucleus upon activation.
Marker
ER Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDIT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 2-5 ug/million cells
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Control
HeLa Whole Cell Lysate
GADD153/CHOP Knockout HeLa Cell Lysate
Control Peptide
CHOP/GADD153 Peptide (NBP2-13172PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-13172 in the following applications:

Publications
Read Publications using
NBP2-13172 in the following applications:

Reactivity Notes

Human, mouse, rat, and primate. Mouse reactivity reported in scientific literature (PMID:32814096).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
1.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GADD153/CHOP Antibody

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 also known as DNA damage-inducible transcript 3 protein (DDIT3), C/EBP-homologous protein (CHOP), CHOP10, and CEBPZ, is a growth arrest and DNA damage inducible gene that encodes a C/EBP-related protein. GADD153 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. GADD153 can be found in the nucleus and its expression is induced by cellular stresses including nutrient deprivation, endoplasmic reticulum stress, and metabolic perturbations. The GADD153 protein functions as a dominant-negative inhibitor that inhibits DNA-binding activity with other C/EBP members, such as C/EBP and LAP (liver activator protein), by forming heterodimers. Fusion of GADD153 and FUS on chromosome 16 or EWSR1 on chromosome 22, induced by translocation, generates chimeric proteins in myxoid liposarcomas, also known as Ewing sarcoma. GADD153 is implicated in adipogenesis and promotes apoptosis in cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, IHC-P

Publications for GADD153/CHOP Antibody (NBP2-13172)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(9)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-13172 Applications Species
Yu Y, Wang F, Wang J et al. Ketogenic diet attenuates aging-associated myocardial remodeling and dysfunction in mice Exp Gerontol Oct 15 2020 [PMID: 32814096] (WB, Mouse) WB Mouse
Kasetti RB, Patel PD, Maddineni P, Zode GS Ex-vivo cultured human corneoscleral segment model to study the effects of glaucoma factors on trabecular meshwork PLoS ONE Jun 24 2020 [PMID: 32579557] (WB, Human) WB Human
Cai H, Han B et al. Metformin attenuates the D galactose induced aging process via the UPR through the AMPK/ERK1/2 signaling pathways. Int J Mol Med Jan 3 2020 [PMID: 31922237] (WB, Rat) WB Rat
Wang X, Peng P, Pan Z et al. Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells Biol. Res. Jul 5 2019 [PMID: 31277690] (WB, Human) WB Human
Sbiera S, kendl S, Weigand I et al. Hsp90 inhibition in adrenocortical carcinoma: Limited drug synergism with mitotane. Mol. Cell. Endocrinol. Oct 10 2018 [PMID: 30315857] (WB, Human) WB Human
Maddineni P, Kasetti RB, Zode GS. Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models. Methods Mol. Biol. 2018 Dec 01 [PMID: 29190024] (Human) Human
Kasetti RB, Maddineni P, Millar JC et al. Increased synthesis and deposition of extracellular matrix proteins leads to endoplasmic reticulum stress in the trabecular meshwork Sci Rep. 2017 Nov 02 [PMID: 29097767] (WB, Human) WB Human
Kunde DA, Chong WC, Nerurkar PV et al. Bitter melon protects against ER stress in LS174T colonic epithelial cells. BMC Complement Altern Med 2017 Jan 03 [PMID: 28049460]
Kasetti RB, Phan TN, Millar JC, Zode GS. Expression of Mutant Myocilin Induces Abnormal Intracellular Accumulation of Selected Extracellular Matrix Proteins in the Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. Nov 1 2016 [PMID: 27820874] (Human) Human
Sbiera S, Leich E, Liebisch G et al. Mitotane inhibits Sterol-O-Acyl Transferase 1 triggering lipid-mediated endoplasmic reticulum stress and apoptosis in adrenocortical carcinoma cells. Endocrinology 2015 Aug 25 [PMID: 26305886] (WB, Human) WB Human
Show All 13 Publications.

Review for GADD153/CHOP Antibody (NBP2-13172) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-13172:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GADD153/CHOP NBP2-13172
Enlarge
5
reviewed by:
Anonymous
IHC-P Mouse 01/23/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLiver tissue
SpeciesMouse
LotA-2

Comments

CommentsCHOP: NBP2-13172
Antigen retreival: Citrate buffer pH=6.0
Blocking: 10% BSA in 0.1%Tween in TBS
Primary antobidy: 1:100 in 1% BSA in 0.1%Tween in TBS
Incubation: overnight at 4 degree
2ndry antibody: 1:200 incubation for 1 hour at room temperature
AB complex: 1:100 for 1hour at room temprature
Develpment. DAB

Product General Protocols

Video Protocols

WB Video Protocol

FAQs for GADD153/CHOP Antibody (NBP2-13172) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 NBP2-13172V
FITC NBP2-13172F

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/23/2020
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol DDIT3
Entrez
Uniprot