Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4)
0.02% Sodium Azide
Immunogen affinity purified
Western Blot 1:1000
This CHOP/GADD153 antibody is useful for Western Blot and IHC-paraffin embedded sections. In Western Blot, bands are seen ~19 and 27 kDa in tunicamycin treated HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse colon tissue which represents the inactivated form of CHOP/GADD153. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Read Publications using NBP2-13172 in the following applications:
Growth arrest and DNA damage-inducible protein GADD153
GADD153 also known as DNA damage-inducible transcript 3 protein (DDIT3), C/EBP-homologous protein (CHOP), CHOP10, and CEBPZ, is a growth arrest and DNA damage inducible gene that encodes a C/EBP-related protein. GADD153 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. GADD153 can be found in the nucleus and its expression is induced by cellular stresses including nutrient deprivation, endoplasmic reticulum stress, and metabolic perturbations. The GADD153 protein functions as a dominant-negative inhibitor that inhibits DNA-binding activity with other C/EBP members, such as C/EBP and LAP (liver activator protein), by forming heterodimers. Fusion of GADD153 and FUS on chromosome 16 or EWSR1 on chromosome 22, induced by translocation, generates chimeric proteins in myxoid liposarcomas, also known as Ewing sarcoma. GADD153 is implicated in adipogenesis and promotes apoptosis in cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.