GADD153/CHOP Antibody

Images

 
Western Blot: GADD153/CHOP Antibody [NBP2-13172] - CHOP/GADD153 Antibody [NBP2-13172] - WB analysis of CHOP/GADD153 in tunicamycin treated (+) and untreated (-) HeLa cell lysate.
Immunohistochemistry: CHOP/GADD153 Antibody [NBP2-13172] - IHC staining of CHOP/GADD153 in mouse colon using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.67 mg/ml
   

Biological Strategies

     

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Catalog# & Conjugate Size Price

GADD153/CHOP Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human CHOP/GADD153 protein (between residues 100-150) [UniProt P35638]
Localization
Cytoplasm when unactivated, translocation to nucleus upon activation.
Marker
ER Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDIT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This CHOP/GADD153 antibody is useful for Western Blot and IHC-paraffin embedded sections. In Western Blot, bands are seen ~19 and 27 kDa in tunicamycin treated HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse colon tissue which represents the inactivated form of CHOP/GADD153. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
HeLa Lysate (NB800-PC1)
Control Peptide
CHOP/GADD153 Peptide (NBP2-13172PEP)
Publications
Read Publications using
NBP2-13172 in the following applications:

  • WB
    4 publications

Reactivity Notes

Human, mouse, rat, and primate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.02% Sodium Azide
Concentration
1.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GADD153/CHOP Antibody

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 also known as DNA damage-inducible transcript 3 protein (DDIT3), C/EBP-homologous protein (CHOP), CHOP10, and CEBPZ, is a growth arrest and DNA damage inducible gene that encodes a C/EBP-related protein. GADD153 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. GADD153 can be found in the nucleus and its expression is induced by cellular stresses including nutrient deprivation, endoplasmic reticulum stress, and metabolic perturbations. The GADD153 protein functions as a dominant-negative inhibitor that inhibits DNA-binding activity with other C/EBP members, such as C/EBP and LAP (liver activator protein), by forming heterodimers. Fusion of GADD153 and FUS on chromosome 16 or EWSR1 on chromosome 22, induced by translocation, generates chimeric proteins in myxoid liposarcomas, also known as Ewing sarcoma. GADD153 is implicated in adipogenesis and promotes apoptosis in cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GADD153/CHOP Antibody (NBP2-13172)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP2-13172 Applications Species
Kunde DA, Chong WC, Nerurkar PV et al. Bitter melon protects against ER stress in LS174T colonic epithelial cells. BMC Complement Altern Med 2017 Jan 03 [PMID: 28049460]
Kasetti RB, Phan TN, Millar JC, Zode GS. Expression of Mutant Myocilin Induces Abnormal Intracellular Accumulation of Selected Extracellular Matrix Proteins in the Trabecular Meshwork. Invest. Ophthalmol. Vis. Sci. Nov 1 2016 [PMID: 27820874] (Human) Human
Sbiera S, Leich E, Liebisch G et al. Mitotane inhibits Sterol-O-Acyl Transferase 1 triggering lipid-mediated endoplasmic reticulum stress and apoptosis in adrenocortical carcinoma cells. Endocrinology 2015 Aug 25 [PMID: 26305886] (WB, Human) WB Human
Lee JW, Park HS, Park SA et al. A Novel Small-Molecule Inhibitor Targeting CREB-CBP Complex Possesses Anti-Cancer Effects along with Cell Cycle Regulation, Autophagy Suppression and Endoplasmic Reticulum Stress PLoS ONE 2015 Apr 22 [PMID: 25897662] (WB, Human) WB Human
Han Y, Yi W, Qin J et al. Carbon monoxide offers neuroprotection from hippocampal cell damage induced by recurrent febrile seizures through the PERK-activated ER stress pathway. Neurosci. Lett. 2015 Jan 12 [PMID: 25434873] (WB, Rat)

Details:
CHOP/GADD153 rabbit polyclonal antibody used at 1:1000 dilution for WB on lysates of rat hippocampal neurons from experimental groups namely - control, FS (rat model of recurrent febrile seizures), FS + ZnPP-IX group (a CO synthase inhibitor), and FS + Hemin (a CO donor).
WB Rat
Nashine S, Liu Y, Kim Bj et al. Role of C/EBP Homologous Protein in Retinal Ganglion Cell Death after Ischemia/Reperfusion Injury. Invest. Ophthalmol. Vis. Sci. 2014 Nov 20 [PMID: 25414185] (WB, Mouse) WB Mouse

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Product General Protocols

Video Protocols

WB Video Protocol

FAQs for GADD153/CHOP Antibody (NBP2-13172) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NBP2-13172V
FITC Labeled NBP2-13172F

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Bioinformatics

Gene Symbol DDIT3
Entrez
Uniprot