GADD153/CHOP Antibody [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 405

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Catalog# & Conjugate Size Price

GADD153/CHOP Antibody [DyLight 405] Summary

Immunogen
A synthetic peptide made to an internal portion of the human CHOP/GADD153 protein (between residues 100-150) [UniProt P35638]
Localization
Cytoplasm when unactivated, translocation to nucleus upon activation.
Marker
ER Stress Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDIT3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This CHOP/GADD153 antibody is useful for Western Blot and IHC-paraffin embedded sections. In Western Blot, bands are seen ~19 and 27 kDa in tunicamycin treated HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse colon tissue which represents the inactivated form of CHOP/GADD153. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human, mouse, rat, and primate.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for GADD153/CHOP Antibody [DyLight 405]

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 also known as DNA damage-inducible transcript 3 protein (DDIT3), C/EBP-homologous protein (CHOP), CHOP10, and CEBPZ, is a growth arrest and DNA damage inducible gene that encodes a C/EBP-related protein. GADD153 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. GADD153 can be found in the nucleus and its expression is induced by cellular stresses including nutrient deprivation, endoplasmic reticulum stress, and metabolic perturbations. The GADD153 protein functions as a dominant-negative inhibitor that inhibits DNA-binding activity with other C/EBP members, such as C/EBP and LAP (liver activator protein), by forming heterodimers. Fusion of GADD153 and FUS on chromosome 16 or EWSR1 on chromosome 22, induced by translocation, generates chimeric proteins in myxoid liposarcomas, also known as Ewing sarcoma. GADD153 is implicated in adipogenesis and promotes apoptosis in cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DDIT3
Entrez