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GABARAP Antibody [Janelia Fluor® 646]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97416AF350
Alexa Fluor 405
NBP1-97416AF405
Alexa Fluor 488
NBP1-97416AF488
Alexa Fluor 532
NBP1-97416AF532
Alexa Fluor 594
NBP1-97416AF594
Alexa Fluor 647
NBP1-97416AF647
Alexa Fluor 700
NBP1-97416AF700
Alexa Fluor 750
NBP1-97416AF750
Biotin
NBP1-97416B
DyLight 350
NBP1-97416UV
DyLight 405
NBP1-97416V
DyLight 488
NBP1-97416G
DyLight 550
NBP1-97416R
DyLight 650
NBP1-97416C
DyLight 680
NBP1-97416FR
DyLight 755
NBP1-97416IR
FITC
NBP1-97416F
HRP
NBP1-97416H
Janelia Fluor 549
NBP1-97416JF549
Unconjugated
NBP1-97416
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GABARAP Antibody [Janelia Fluor® 646] Summary

Immunogen
A synthetic peptide made to an N-terminal region of the human GABARAP protein (within residues 1-50). [Swiss-Prot O95166]
Localization
Endomembrane system. Cytoplasm. Golgi apparatus membrane. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABARAP
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GABARAP Antibody [Janelia Fluor® 646]

  • Apg8p1
  • ATG8A
  • FLC3B
  • FLJ25768
  • GABA(A) receptor-associated proteinMGC120154
  • GABARAP
  • gamma-aminobutyric acid receptor-associated protein
  • MM46
  • MM46MGC120155

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows no positivity in hepatocytes as expected.
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NBP2-03449
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p62/SQSTM1
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H00008878-M01
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Species: Hu, Mu, Rt, Bv, Ha, Rb
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(1F12-1B5)
H00002965-M01
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NBP1-31381
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dynactin 4
Antibody (3G9D7)
NBP2-37399
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NCOA6 Antibody
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SAM68 Antibody
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Glutamine
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Calreticulin
Antibody
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ATG7 Antibody
(683906) [Unconjugate
MAB6608
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATG7 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ATG7 at approximately 72 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse ATG7 Monoclonal Antibody (Catalog # MAB6608). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
(4 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97416AF350
Alexa Fluor 405 NBP1-97416AF405
Alexa Fluor 488 NBP1-97416AF488
Alexa Fluor 532 NBP1-97416AF532
Alexa Fluor 594 NBP1-97416AF594
Alexa Fluor 647 NBP1-97416AF647
Alexa Fluor 700 NBP1-97416AF700
Alexa Fluor 750 NBP1-97416AF750
Biotin NBP1-97416B
DyLight 350 NBP1-97416UV
DyLight 405 NBP1-97416V
DyLight 488 NBP1-97416G
DyLight 550 NBP1-97416R
DyLight 650 NBP1-97416C
DyLight 680 NBP1-97416FR
DyLight 755 NBP1-97416IR
FITC NBP1-97416F
HRP NBP1-97416H
Janelia Fluor 549 NBP1-97416JF549
Janelia Fluor 646 NBP1-97416JF646
Learn about Custom Antibody Labeling

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Check out the latest blog posts on GABARAP.

  Read full blog post.
Breakdown: Interpreting LC3 Antibody WB Results
In rodents, MAP1LC3 (Microtubule-associated protein 1 light chain 3) is expressed in the renal visceral epithelial cells, or podocytes. LC3 antibody analysis has shown the protein accumulates in its membrane-bound form, LC3II, following conversio...  Read full blog post.
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Bioinformatics

Gene Symbol GABARAP