GABA-AR alpha 6 Antibody

Immunohistochemistry-Paraffin: GABA-A R alpha 6 Antibody [NBP2-26069] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GABA-AR alpha 6 Antibody Summary

• Description: Novus Biologicals Goat GABA-AR alpha 6 Antibody (NBP2-26069) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-GABA-AR alpha 6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence ENALGKLEVEGN-C corresponding to N-Terminus according to NP_000802.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GABRA6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave bands at approx. 75 kDa band and 37 kDa band in human cerebellum lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.0 kDa band according to NP_000802.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GABA-AR alpha 6 Antibody

• GABA A R alpha 6
• GABA subunit A receptor alpha 6
• GABA(A) receptor subunit alpha-6
• GABAAR alpha 6
• GABAARa6
• GABRA6
• gamma-aminobutyric acid (GABA) A receptor, alpha 6
• gamma-aminobutyric acid receptor subunit alpha-6
• MGC116903
• MGC116904

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. The alpha 6 subunit is expressed in cerebellar granule cells. Though it is concentrated at synapses, it is found on extrasynaptic membranes, as well.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 6 Antibody (NBP2-26069)(1)

Publication using NBP2-26069

• Green EK, Grozeva D, Moskvina V et al. Variation at the GABAA receptor gene, Rho 1 (GABRR1) associated with susceptibility to bipolar schizoaffective disorder. Am J Med Genet B Neuropsychiatr Genet 2010-10-05 [PMID: 20583128]

Bioinformatics

• Gene Symbol: GABRA6
• Entrez:
  • 2559(Human)