| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S415-24 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 488 |
| Immunogen | Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5. |
| Localization | Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane |
| Specificity | Detects approx 30kDa. No cross-reactivity against KChIP2, KChIP3, or KChIP4. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GABRA5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![GABA-AR alpha 5 Antibody (S415-24) [DyLight 488]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GABA-AR alpha 5 Antibody (S415-24) [DyLight 488]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 405 | NBP2-59700AF405 | |
| Alexa Fluor 488 | NBP2-59700AF488 | |
| Alexa Fluor 647 | NBP2-59700AF647 | |
| Alexa Fluor 700 | NBP2-59700AF700 | |
| Biotin | NBP2-59700B | |
| DyLight 350 | NBP2-59700UV | |
| DyLight 405 | NBP2-59700V | |
| DyLight 488 | NBP2-59700G | |
| DyLight 550 | NBP2-59700R | |
| DyLight 650 | NBP2-59700C | |
| DyLight 680 | NBP2-59700FR | |
| DyLight 755 | NBP2-59700IR | |
| FITC | NBP2-59700F | |
| HRP | NBP2-59700H | |
| Janelia Fluor 549 | NBP2-59700JF549 | |
| Janelia Fluor 646 | NBP2-59700JF646 |
Diseases for GABA-AR alpha 5 Antibody (NBP2-59700G)Discover more about diseases related to GABA-AR alpha 5 Antibody (NBP2-59700G).
| Pathways for GABA-AR alpha 5 Antibody (NBP2-59700G)View related products by pathway.
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PTMs for GABA-AR alpha 5 Antibody (NBP2-59700G)Learn more about PTMs related to GABA-AR alpha 5 Antibody (NBP2-59700G).
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| Gene Symbol | GABRA5 |
![Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.](http://images.novusbio.com/images2/GABA-A-R-beta-3-Antibody-Immunofluorescence-NB300-199-img0006.jpg)
![Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Western blot of whole-olfactory bulb homogenates using the B3-subunit antibody in rAAV-Cre-injected versus non-injected GABRB3loxP/loxP mice (each six samples from three mice). The B3-subunit appears as a band of 55 kDa, consistent with its predicted molecular weight. Densitometric analysis (mean+/-s.e.m.) revealed a significant difference (t-test; *P=0.016). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9950) licensed under a CC-BY licence.](http://images.novusbio.com/images2/GABA-A-R-beta-3-Antibody-Western-Blot-NB300-199-img0010.jpg)
![Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Each channel was filtered frame by frame. The raw data was acquired at high confocal resolution (XY: 234.32 um x 234.32 um, pixel size = 0.114 um) using a 63x glycerol objective (NA=1.3). Z-steps were 0.15 um per frame. Acquired raw data (1) was filtered using an anisotropic diffusion 2D filter (2). (5). The final images (6) were obtained by thresholding the immunosignal. Scale bars: 10um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9950) licensed under a CC-BY licence.](http://images.novusbio.com/images2/GABA-A-R-beta-3-Antibody-Immunohistochemistry-NB300-199-img0011.jpg)
![Western Blot: GABA-A R gamma 3 Antibody [NB100-56662] - Analysis of GABAA Rg3 in 15 ugs of rat brain cell lysate using this antibody.](http://images.novusbio.com/images2/GABA-A-R-gamma-3-Antibody-Western-Blot-NB100-56662-img0003.jpg)
![Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.](http://images.novusbio.com/images2/GABA-A-R-alpha-1-Antibody-Western-Blot-NB300-191-img0003.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Comparative proteome analysis of 3xTg-AD and control samples at different stages of AD. Immunoblot analysis of most regulated hits. Soluble fractions of brain proteins were analyzed from four 2-month-old control and 3xTg-AD mice animals, respectively. Hebp1 and Glo1 levels were consistently elevated in the transgenic animals as compared to wild type controls. Ca1 levels were reduced in transgenic animals. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/47498), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Carbonic Anhydrase I-CA1 Antibody-Western Blot-NBP1-88191-img0015.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/images2/Carbonic-Anhydrase-I-CA1-Antibody-Immunohistochemistry-Paraffin-NBP1-88191-img0014.jpg)
![Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.](http://images.novusbio.com/images2/Carbonic-Anhydrase-I-CA1-Antibody-Western-Blot-NBP1-88191-img0006.jpg)
![SDS-Page: Recombinant Human OCA2 Protein [H00004948-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00004948-Q01-1.jpg)
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.](http://images.novusbio.com/images2/E6AP-UBE3A Antibody (2F6)-Western Blot-H00007337-M01-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/images2/E6AP-UBE3A-Antibody-(2F6)-Immunocytochemistry-Immunofluorescence-H00007337-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/E6AP-UBE3A-Antibody-(2F6)-Immunohistochemistry-Paraffin-H00007337-M01-img0012.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.](http://images.novusbio.com/images2/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-151-img0007.jpg)
![Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.](http://images.novusbio.com/images2/GABA-A-R-gamma-2-Antibody-Immunohistochemistry-Paraffin-NB300-151-img0005.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.](http://images.novusbio.com/images2/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-151-img0006.jpg)
![Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/images2/GABA-A-R-beta-1-Antibody-(S96-55)-Western-Blot-NBP1-48319-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive.](http://images.novusbio.com/images2/GABA-A-R-beta-1-Antibody-(S96-55)-Immunocytochemistry-Immunofluorescence-NBP1-48319-img0003.jpg)
![Western Blot: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - CA3 monoclonal antibody (M02), clone 4A12-1A3 Analysis of CA3 expression in K-562.](http://images.novusbio.com/images2/Carbonic-Anhydrase-III-CA3-Antibody-(4A12-1A3)-Western-Blot-H00000761-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - Analysis of monoclonal antibody to CA3 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/Carbonic-Anhydrase-III-CA3-Antibody-(4A12-1A3)-Immunohistochemistry-Paraffin-H00000761-M02-img0008.jpg)
![Sandwich ELISA: Carbonic Anhydrase III/CA3 Antibody (4A12-1A3) [H00000761-M02] - Detection limit for recombinant GST tagged CA3 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/images2/Carbonic-Anhydrase-III-CA3-Antibody-(4A12-1A3)-Sandwich-ELISA-H00000761-M02-img0009.jpg)
![Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.](http://images.novusbio.com/images2/MeCP2 Antibody-Western Blot-NB600-1101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/images2/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1101-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB600-1101] - Staining in the nucleus of rat brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/images2/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB600-1101-img0005.jpg)
![Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NBP1-31386-img0017.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.](http://images.novusbio.com/images2/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0013.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.](http://images.novusbio.com/images2/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0011.jpg)
![Western Blot: SNRPN Antibody [NBP2-20439] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/images2/SNRPN Antibody-Western Blot-NBP2-20439-img0002.jpg)
![Immunohistochemistry-Paraffin: SNRPN Antibody [NBP2-20439] - SNRPN antibody detects SNRPN protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPN antibody dilution: 1:500.](http://images.novusbio.com/images2/SNRPN-Antibody-Immunohistochemistry-Paraffin-NBP2-20439-img0004.jpg)
![Western Blot: SNRPN Antibody [NBP2-20439] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/SNRPN Antibody-Western Blot-NBP2-20439-img0001.jpg)
![Western Blot: B3GALNT1 Antibody [NBP2-92677] - Analysis of extracts of various cell lines, using B3GALNT1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/images2/B3GALNT1 Antibody-Western Blot-NBP2-92677-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: B3GALNT1 Antibody [NBP2-92677] - Analysis of U2OS cells using B3GALNT1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/B3GALNT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92677-img0001.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.](http://images.novusbio.com/images2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.](http://images.novusbio.com/images2/GAPDH-Antibody-(1D4)-Immunocytochemistry-Immunofluorescence-NB300-221-img0009.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.](http://images.novusbio.com/images2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0008.jpg)
![Western Blot: UROD Antibody [NBP2-26190] - Staining of K562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/UROD-Antibody-Western-Blot-NBP2-26190-img0001.jpg)
![Immunohistochemistry-Paraffin: UROD Antibody [NBP2-26190] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/UROD-Antibody-Immunohistochemistry-Paraffin-NBP2-26190-img0002.jpg)
![Western Blot: GABA A R alpha 3 Antibody [NBP1-87499] - Analysis in control (vector only transfected HEK293T lysate) and GABRA3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/GABA-A-R-alpha-3-Antibody-Western-Blot-NBP1-87499-img0012.jpg)
![Immunohistochemistry: GABA A R alpha 3 Antibody [NBP1-87499] - Staining of mouse thalamus shows strong positivity in the paraventricular thalamic nucleus.](http://images.novusbio.com/images2/GABA-A-R-alpha-3-Antibody-Immunohistochemistry-NBP1-87499-img0007.jpg)
![Western Blot: GABA A R alpha 3 Antibody [NBP1-87499] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/images2/GABA-A-R-alpha-3-Antibody-Western-Blot-NBP1-87499-img0010.jpg)