GABA-AR alpha 5 Antibody (S415-24) - BSA Free

Western Blot: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 5 protein using Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody, Clone S415-24 . Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 5 Monoclonal Antibody at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa.

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody, Clone S415-24 (NBP2-59700). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-AR alpha 5 Alpha 5 Monoclonal Antibody (NBP2-59700) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-AR alpha 5 Alpha 5 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: GABA-AR alpha 5 Antibody (S415-24) [NBP2-59700] - Analysis using Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody, Clone S415-24 . Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha5 Antibody (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S415-24
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GABA-AR alpha 5 Antibody (S415-24) - BSA Free Summary

• Description: Novus Biologicals Mouse GABA-AR alpha 5 Antibody (S415-24) - BSA Free (NBP2-59700) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GABA-AR alpha 5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
• Localization: Cell Junction , Synapse , Postsynaptic Cell Membrane , Cell Membrane
• Specificity: Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000
• Application Notes: A 1:100 dilution of this antibody was sufficient for detection of GABA-A R, Alpha5 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for GABA-AR alpha 5 Antibody (S415-24) - BSA Free

• GABA A R alpha 5
• GABA(A) receptor subunit alpha-5
• GABAAR alpha 5
• GABAARa5
• GABRA5
• gamma-aminobutyric acid (GABA) A receptor, alpha 5
• gamma-aminobutyric acid receptor subunit alpha-5
• MGC138184

Background

The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 5 Antibody (NBP2-59700)(1)

Publication using NBP2-59700

• Cai X, Qiu L, Wang C et al. Hippocampal Inhibitory Synapses Deficits Induced by alpha 5-containing GABAA Receptors Mediates Chronic Neuropathic Pain-related Cognitive Impairment Mol Neurobiol 2022-07-18 [PMID: 35849280]

Bioinformatics

• Gene Symbol: GABRA5