GABA-AR alpha 5 Antibody (5C11) - BSA Free

Immunohistochemistry: GABA-AR alpha 5 Antibody (5C11) [NBP3-26561] - Image of GABA-AR alpha 5 Antibody (5C11) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: GABA-AR alpha 5 Antibody (5C11) [NBP3-26561] - Positive Western Blot detected in: Ntera-2 whole cell lysate, HEK293 whole cell lysate. All lanes: GABA-AR alpha 5 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53 kDa Observed band size: 70 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 5C11
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GABA-AR alpha 5 Antibody (5C11) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human GABA-AR alpha 5 [UniProt P31644]
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GABRA5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GABA-AR alpha 5 Antibody (5C11) - BSA Free

• GABA A R alpha 5
• GABA(A) receptor subunit alpha-5
• GABAAR alpha 5
• GABAARa5
• GABRA5
• gamma-aminobutyric acid (GABA) A receptor, alpha 5
• gamma-aminobutyric acid receptor subunit alpha-5
• MGC138184

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRA5