GABA-AR alpha 4 Antibody

Western Blot: GABA-A R alpha 4 Antibody [NBP1-18844] - Analysis of GABA A Receptor alpha 4 in Human Frontal Cortex lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: GABA-A R alpha 4 Antibody [NBP1-18844] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GABA-AR alpha 4 Antibody Summary

• Description: Novus Biologicals Goat GABA-AR alpha 4 Antibody (NBP1-18844) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-GABA-AR alpha 4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EKAKRKTSKPPQE corresponding to internal region according to NP_000800.2.
• Epitope: internal region
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GABRA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 61.6 kDa band according to NP_000800.2). IHC-P: Human cortex shows patchy staining near or at the neuronal cell membrane.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GABA-AR alpha 4 Antibody

• GABA A R alpha 4
• GABA(A) receptor subunit alpha-4
• GABAAR alpha 4
• GABAARa4
• GABRA4
• gamma-aminobutyric acid (GABA) A receptor, alpha 4
• gamma-aminobutyric acid receptor subunit alpha-4

Background

GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 4 Antibody (NBP1-18844)(1)

Publication using NBP1-18844

• Ma DQ, Whitehead PL, Menold MM et al. Identification of significant association and gene-gene interaction of GABAreceptor subunit genes in autism. Am J Hum Genet 2005-09-01 [PMID: 16080114]

Bioinformatics

• Gene Symbol: GABRA4
• Entrez:
  • 2557(Human)
• Uniprot:
  • NP_000800.2(Human)