GABA-AR alpha 1 Antibody (1F4) - Azide and BSA Free

• Reactivity: Hu
• Applications: ICC/IF, WB
• Clone: 1F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

GABA-AR alpha 1 Antibody (1F4) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised against alpha 1 beta 2 gamma 2 GABA-A receptor.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABRA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • X-Ray Crystallography
• Application Notes: FSEC: High affinity and specificity of the 1F4 antibody to alpha 1 beta 2 gamma 2 GABA-A receptor was demonstrated using fluorescence-detection size exclusion chromatography with GFP-tagged receptor (shift in elution volume) (Zhu et al., 2018). Crystallography: Furthermore, this antibody was utilised in production and purification of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018). Finally, Fab fragments derived from the antibody were used to facilitate crystallisation and improve stabilisation of the alpha 1 beta 2 gamma 2 GABA-A receptor (Zhu et al., 2018).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GABA-AR alpha 1 Antibody (1F4) - Azide and BSA Free

• ECA4
• EJM
• EJM5GABA(A) receptor subunit alpha-1
• GABA A R alpha 1
• GABAAR alpha 1
• GABAARa1
• GABRA1
• gamma-aminobutyric acid (GABA) A receptor, alpha 1
• gamma-aminobutyric acid receptor subunit alpha-1

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABRA1