GABA-A R gamma 2 Antibody

Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.

Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.

Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.

• Reactivity: Hu, Mu, Rt, Tr, Ze, Hu, Bv, Ch, Pm, Ze
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GABA-A R gamma 2 Antibody Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH. Accession # P18508
• Localization: Cell Membrane
• Specificity: Specific for endogenous levels of the ~46 kDa gamma 2-subunit of the GABAA receptor.
• Predicted Species: Human (100%), Chicken (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GABRG2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24480790). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28285877).
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16807363). Human reactivity reported in scientific literature (PMID: 24480790). Turkey reactivity reported in scientific literature (PMID: 27055929). Zebrafish reactivity reported in scientific literature (PMID: 28285877).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for GABA-A R gamma 2 Antibody

• CAE2
• ECA2
• GABA A R gamma 2
• GABA(A) receptor subunit gamma-2
• GABAAR gamma 2
• GABAARg2
• GABRG2
• gamma-aminobutyric acid (GABA) A receptor, gamma 2
• gamma-aminobutyric acid receptor subunit gamma-2
• GEFSP3

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl(-) channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-A R gamma 2 Antibody (NB300-151)(10)

Publications using NB300-151

• Su M, Xuan E, Sun X, Pan G et Al. Synaptic adhesion molecule protocadherin-?C5 mediates ?-amyloid-induced neuronal hyperactivity and cognitive deficits in Alzheimer's disease J Neurochem 2024-02-03 [PMID: 38308496]
• Li S, Jiang X, Wu Q et al. Electroacupuncture Suppresses CCI-Induced Neuropathic Pain through GABAA Receptors Evidence-based complementary and alternative medicine : eCAM 2022-10-07 [PMID: 36248405] (WB, Rat)
• Rao MB, Didiano D, Patton JG. Neurotransmitter-Regulated Regeneration in the Zebrafish Retina. Stem Cell Reports. 2017-03-06 [PMID: 28285877] (IHC-Fr, Zebrafish)
• Kosonsiriluk S, Chaiworakul V, Mauro LJ, El Halawani ME. Enhanced GABAergic inhibition in the premammillary nucleus of photorefractory turkey hens via GABAA receptor upregulation. Gen. Comp. Endocrinol. 2016-04-04 [PMID: 27055929] (IF/IHC, Turkey)
• Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007-01-01 (WB, Mouse)
• Kang Yunhee, Ge Yuan, Cassidy Robert M et al. A combined transgenic proteomic analysis and regulated trafficking of neuroligin-2. J Biol Chem. 2014-10-17 [PMID: 25190809]
• Ishii A, Kanaumi T, Sohda M et al. Association of nonsense mutation in GABRG2 with abnormal trafficking of GABAA receptors in severe epilepsy. Epilepsy Research 2014-03-01 [PMID: 24480790] (ICC/IF, IHC-P, Human, Mouse)
• Borghese CM, Werner DF, Topf N et al. An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther. 2006-10-01 [PMID: 16807363] (WB, Mouse)
• Hamilton K. Basic Helix Loop Helix Enhancer 40 in Neuronal Excitability and Synaptic Plasticity Dissertation 2016-01-01 (KO, WB, Mouse)
• Tamargo-GOmez I, MartInez-GarcIa GG, SuArez MF et al. ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration Cell death and differentiation 2021-04-01 [PMID: 33795848]

FAQs for GABA-A R gamma 2 Antibody (NB300-151). (Showing 1 - 2 of 2 FAQs).

1. I am looking for a "Primary antibody for GABAa receptor gamma 2" raised from antigen whose aa sequence is between exon 5-10. The application will be for "Immunohistochemistry". I would like to know if you have a product to match it?
   • NB300-190 is direceted against GABA A receptor gamma 2, is suitable for use in IHC and uses a fusion protein from the cytoplasmic loop of the gamma 2 subunit as an antigen, so this should suit your needs.
2. Are there neutralizing peptides for this antibody?
   • There is no neutralizing peptide available for this antibody.

Bioinformatics

• Gene Symbol: GABRG2
• Entrez:
  • 2566(Human)
  • 14406(Mouse)
  • 29709(Rat)
• Uniprot:
  • P22723(Mouse)
  • P18508(Rat)