GABA-A R gamma 2 Antibody

Images

 
Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.
Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.
Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.

Product Details

Summary
Reactivity Hu, Mu, Rt, Tr, Ze, Bv, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GABA-A R gamma 2 Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH
Localization
Cell Membrane
Specificity
Specific for the ~46k gamma2-subunit of the GABAA receptor in Western blots of rat brain extracts.
Predicted Species
Bovine (100%), Chicken (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRG2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
  • Knockout Validated
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24480790). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28285877).
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB300-151 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16807363). Human reactivity reported in scientific literature (PMID: 24480790). Turkey reactivity reported in scientific literature (PMID: 27055929). Zebrafish reactivity reported in scientific literature (PMID: 28285877).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for GABA-A R gamma 2 Antibody

  • CAE2
  • ECA2
  • GABA A R gamma 2
  • GABA(A) receptor subunit gamma-2
  • GABAAR gamma 2
  • GABAARg2
  • GABRG2
  • gamma-aminobutyric acid (GABA) A receptor, gamma 2
  • gamma-aminobutyric acid receptor subunit gamma-2
  • GEFSP3

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl(-) channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-151
Species: Hu, Mu, Rt, Tr, Ze, Bv, Ch, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, KO

Publications for GABA-A R gamma 2 Antibody (NB300-151)(7)

We have publications tested in 4 confirmed species: Human, Mouse, Turkey, Zebrafish.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-FR, IHC-P, KO, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-FR
(1)
IHC-P
(1)
KO
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Turkey
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB300-151 Applications Species
Rao MB, Didiano D, Patton JG. Neurotransmitter-Regulated Regeneration in the Zebrafish Retina. Stem Cell Reports. Mar 6 2017 [PMID: 28285877] (IHC-FR, Zebrafish) IHC-FR Zebrafish
Hamilton K. Basic Helix Loop Helix Enhancer 40 in Neuronal Excitability and Synaptic Plasticity Dissertation 2016 (WB, KO, Mouse) WB, KO Mouse
Borghese CM, Werner DF, Topf N, Baron NV, Henderson LA, Boehm SL 2nd, Blednov YA, Saad A, Dai S, Pearce RA, Harris RA, Homanics GE, Harrison NL. An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther;319(1):208-18. 2006 Oct. [PMID: 16807363] (WB, Mouse) WB Mouse
Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007 (WB, Mouse) WB Mouse
Kosonsiriluk S, Chaiworakul V, Mauro LJ, El Halawani ME. Enhanced GABAergic inhibition in the premammillary nucleus of photorefractory turkey hens via GABAA receptor upregulation. Gen. Comp. Endocrinol. 2016 Apr 04 [PMID: 27055929] (IHC, Turkey) IHC Turkey
Kang Y, Ge Y, Cassidy RM et al. A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2. J Biol Chem. 2014 Sep 04 [PMID: 25190809]
Ishii A, Kanaumi T, Sohda M et al. Association of nonsense mutation in GABRG2 with abnormal trafficking of GABAA receptors in severe epilepsy. Epilepsy Research 2014 Mar 01 [PMID: 24480790] (IHC-P, ICC/IF, Human, Mouse) IHC-P, ICC/IF Human, Mouse

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Product General Protocols

View specific protocols for GABA-A R gamma 2 Antibody (NB300-151): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GABA-A R gamma 2 Antibody (NB300-151). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for a "Primary antibody for GABAa receptor gamma 2" raised from antigen whose aa sequence is between exon 5-10. The application will be for "Immunohistochemistry". I would like to know if you have a product to match it?
    • NB300-190 is direceted against GABA A receptor gamma 2, is suitable for use in IHC and uses a fusion protein from the cytoplasmic loop of the gamma 2 subunit as an antigen, so this should suit your needs.
  2. Are there neutralizing peptides for this antibody?
    • There is no neutralizing peptide available for this antibody.

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Bioinformatics

Gene Symbol GABRG2
Entrez
Uniprot