Home » GABA-A R delta » GABA-A R delta Antibodies » GABA-A R delta Antibody (S151-3) [DyLight 550]

GABA-A R delta Antibody (S151-3) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S151-3
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-47616AF405
Alexa Fluor 488
NBP1-47616AF488
Alexa Fluor 647
NBP1-47616AF647
Alexa Fluor 700
NBP1-47616AF700
Biotin
NBP1-47616B
DyLight 350
NBP1-47616UV
DyLight 405
NBP1-47616V
DyLight 488
NBP1-47616G
DyLight 650
NBP1-47616C
DyLight 680
NBP1-47616FR
DyLight 755
NBP1-47616IR
FITC
NBP1-47616F
HRP
NBP1-47616H
Janelia Fluor 549
NBP1-47616JF549
Janelia Fluor 646
NBP1-47616JF646
Unconjugated
NBP1-47616
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GABA-A R delta Antibody (S151-3) [DyLight 550] Summary

Immunogen
Synthetic peptide amino acids 15-34 of rat GABA-A-R-Delta
Localization
Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane
Specificity
Detects approx 55kDa.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GABRD
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for GABA-A R delta Antibody (S151-3) [DyLight 550]

  • EIG10
  • EJM7
  • GABA A R delta
  • GABA(A) receptor subunit delta
  • GABA-A receptor, delta polypeptide
  • GABAAR delta
  • GABAARd
  • GABRD
  • gamma-aminobutyric acid (GABA) A receptor, delta
  • gamma-aminobutyric acid receptor subunit delta
  • GEFSP5
  • MGC45284

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.
Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.
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Western Blot: SCN1B Antibody [NBP2-94559] - Analysis of extracts of various cell lines, using SCN1B at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Species: Hu, Mu, Rt
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Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.
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Immunocytochemistry/Immunofluorescence: FMRP Antibody (1C6) [NBP2-01770] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FMRP.
Immunohistochemistry-Paraffin: FMRP Antibody (1C6) [NBP2-01770] - Staining of paraffin-embedded Human pancreas tissue using anti-FMRP mouse monoclonal antibody.
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(1 Publication)
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Western Blot: Prostaglandin E Synthase Antibody [NBP1-87852] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: Prostaglandin E Synthase Antibody [NBP1-87852] - Staining of human cell line SiHa shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Prostaglandin E Synthase Antibody [NBP1-87852] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
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Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: Jak2 Antibody (53B7) [NBP2-59451] - Human breast cancer MCF7 cells were treated with control (Con) or Adenosine (Ado) and the expression of JAK2 was detected. Wester blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) and anti-human JAK2 antibody at 1:100 with goat anti-mouse IgG Alexa Fluor 488 conjugate (Green).
Flow Cytometry: Jak2 Antibody (53B7) [NBP2-59451] - Analysis of JAK2 in HeLa cell line, antibody at 2 - 5 ug per 10^6cells (red line). Secondary antibody: goat anti-mouse IgG Alexa Fluor 488 conjugate. Isotype control antibody: mouse IgG (black line).
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Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lane R), pig (Lane P), bovine (Lane B), or human (Lane Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.
Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NB110-41404] - Immunofluorescence using NB110-41404. Submitted via verified customer review.
Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction. Nuclei in IF experiments (A-D) were stained with DAPI (cyan), and with nuclear fast red in E and F. At low magnification, anti-glutamine synthase reacted with a single population of cells extending from the ganglion cell layer through the inner nuclear layer. No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).
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NB300-199
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Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Each channel was filtered frame by frame. The raw data was acquired at high confocal resolution (XY: 234.32 um x 234.32 um, pixel size = 0.114 um) using a 63x glycerol objective (NA=1.3). Z-steps were 0.15 um per frame. Acquired raw data (1) was filtered using an anisotropic diffusion 2D filter (2). (5). The final images (6) were obtained by thresholding the immunosignal. Scale bars: 10um. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms9950) licensed under a CC-BY licence.
(2 Reviews)
(10 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
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Tyrosine
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Immunohistochemistry: Tyrosine Hydroxylase Antibody [NB300-109] - Tyrosine hydroxylase immunoreactivity in the central complex and the lateral accessory lobe. A-D: Frontal sections (immunoperoxidase preparations, dorsal to the top). E: Horizontal section (immunofluorescent preparation, posterior to the top). Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0160531), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NB300-109] - Dopamine neurons in the mouse substantia nigra. ICC/IF image submitted by a verified customer review.
Simple Western: Tyrosine Hydroxylase Antibody [NB300-109] - Simple Western lane view shows a specific band for Tyrosine Hydroxylase in 0.2 mg/mL of PC-12 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Western Blot: GBGT1 Antibody [NBP2-14040] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: GBGT1 Antibody [NBP2-14040] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
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Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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SOCS-3 Antibody
(OTI3D3)
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Western Blot: SOCS3 Antibody (3D3) [NBP2-00817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOCS3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOCS3.
Immunocytochemistry/Immunofluorescence: SOCS3 Antibody (3D3) [NBP2-00817] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SOCS3.
Immunohistochemistry-Paraffin: SOCS3 Antibody (3D3) [NBP2-00817] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SOCS3 mouse monoclonal antibody.
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TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
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GABA-A R delta
Antibody (S151-3) [Dy ...
NBP1-47616R
GABA-A R delta Antibody (S151-3) [DyLight 550]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-47616AF405
Alexa Fluor 488 NBP1-47616AF488
Alexa Fluor 647 NBP1-47616AF647
Alexa Fluor 700 NBP1-47616AF700
Biotin NBP1-47616B
DyLight 350 NBP1-47616UV
DyLight 405 NBP1-47616V
DyLight 488 NBP1-47616G
DyLight 550 NBP1-47616R
DyLight 650 NBP1-47616C
DyLight 680 NBP1-47616FR
DyLight 755 NBP1-47616IR
FITC NBP1-47616F
HRP NBP1-47616H
Janelia Fluor 549 NBP1-47616JF549
Janelia Fluor 646 NBP1-47616JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol GABRD
Entrez
Uniprot