Cytohesin-1 Antibody (6G11)

Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human placenta tissue.

Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human tonsil tissue.

Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human breast carcinoma.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clone: 6G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cytohesin-1 Antibody (6G11) Summary

• Immunogen: Purified, His-tagged cytohesin-1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CYTH1
• Purity: PEG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200 - 500
  • Immunohistochemistry-Paraffin 1:200 - 500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1.0 ug/ml
• Application Notes: WB: Detects an approx. 48 kDa band representing both cytohesin-1 and cytohesin-2. IP: Only precipitates cytohesin-2.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: PEG purified

Alternate Names for Cytohesin-1 Antibody (6G11)

• B2-1
• CYTH1
• cytoadhesin 1
• cytohesin 1
• Cytohesin1
• Cytohesin-1
• D17S811ECYTOHESIN-1
• FLJ34050
• FLJ41900
• homolog of secretory protein SEC7
• PH, SEC7 and coiled-coil domain-containing protein 1
• pleckstrin homology, Sec7 and coiled-coil domains 1
• PSCD1
• SEC7 homolog B2-1
• SEC7

Background

Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The PSCD1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. PSCD1 protein is 83% homologous to PSCD2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CYTH1
• Entrez:
  • 9267(Human)
• Uniprot:
  • Q15438()