G-CSFR/CD114 Antibody (PSH03-44)

Western Blot: G-CSFR/CD114 Antibody (PSH03-44) [NBP3-32378] - Western blot analysis of G-CSFR/CD114 on different lysates with Rabbit anti-G-CSFR/CD114 antibody (NBP3-32378) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: THP-1 cell lysate (no heat) (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Ramos cell lysate (20 ug/Lane) Lane 6: Mouse placenta tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (no heat) (40 ug/Lane) Lane 8: Mouse testis tissue lysate (40 ug/Lane) Lane 9: Rat placenta tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (no heat) (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Lane 12: Rat skin tissue lysate (no heat) (40 ug/Lane) Predicted band size: 92 kDa Observed band size: 50-110 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: PSH03-44
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

G-CSFR/CD114 Antibody (PSH03-44) Summary

• Description: Novus Biologicals Rabbit G-CSFR/CD114 Antibody (PSH03-44) (NBP3-32378) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human G-CSFR/CD114 aa 651-836 / 836. (Uniprot: Q99062)
• Localization: Cell membrane; Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CSF3R
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for G-CSFR/CD114 Antibody (PSH03-44)

• CD114 antigen
• CD114
• colony stimulating factor 3 receptor (granulocyte)
• CSF3R
• Csfgr
• G-CSF R
• G-CSF receptor
• GCSFR
• G-CSFR
• GCSFRG-CSF-R
• granulocyte colony-stimulating factor receptor

Background

GCSF Receptor is encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSF3R