G-CSF Antibody (SPM468)

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Immunohistochemistry-Paraffin: G-CSF Antibody (SPM468) [NBP2-44797] - Human Tonsil stained with G-CSF Monoclonal Antibody (SPM468).

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications Flow, IHC-P, ICC/IF
Clone
SPM468
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

G-CSF Antibody (SPM468) Summary

Immunogen
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes
Localization
Cytoplasmic
Specificity
This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF s, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF s share 73% sequence identity at the amino acid level.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CSF3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 17q21.1
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Macaque Monkey, Others not known.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for G-CSF Antibody (SPM468)

  • C17orf33
  • chromosome 17 open reading frame 33
  • colony stimulating factor 3 (granulocyte)
  • CSF3
  • CSF3OS
  • Filgrastim
  • GCSF
  • G-CSF
  • GCSFlenograstim
  • granulocyte colony-stimulating factor
  • Lenograstim
  • MGC45931
  • pluripoietin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CSF3
Entrez
Uniprot