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414-CS) stimulates proliferation in the NFS-60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (125 pg/mL) is neutralized (green line) by increasing concentrations of Mouse G-CSF Monoclonal Antibody (Catalog # MAB414). The ND50 is typically 0.01-0.03 µg/mL." class="big_lightbox" target="_blank">
414-CS) was serially diluted and captured by Rat Anti-Mouse G‑CSF Monoclonal Antibody (Catalog # MAB414) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse G‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
214-CS) and Recombinant Mouse G-CSF (414-CS). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for G-CSF at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
214-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA). The ND50 is typically 8-48 ng/mL." class="big_lightbox" target="_blank">
214-CS) was serially diluted and captured by Mouse Anti-Human G‑CSF Monoclonal Antibody (Catalog # MAB214) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human G‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-214-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
414-CS) stimulates proliferation in the NFS-60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA). The ND50 is typically 0.0025-0.0075 µg/mL." class="big_lightbox" target="_blank">
MAB414R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse G-CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
414-CS) was serially diluted and captured by Rat Anti-Mouse G‑CSF Monoclonal Antibody (Catalog # MAB414) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse G‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-414-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Granulocyte Colony-Stimulating Factor (G-CSF , filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is a 18.7 kDa glycoprotein containing 174 amino acid residues.
![Neutralization G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/images/antibody/G-CSF_MAB414_Block_Neutralize_9188.jpg)
![Western Blot G-CSF Antibody [Unconjugated]](http://images.novusbio.com/images/af-214-na_human-g-csf-affinity-purified-polyclonal-ab-western-blot-639184425510846211.jpg)
![Neutralization G-CSF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/G-CSF_AF-414-NA_Block_Neutralize_8708.jpg)
![Sandwich ELISA G-CSF Antibody Pair [HRP]](http://images.novusbio.com/fullsize/G-CSF-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79432-img0001.jpg)
![N/A G-CSF [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_GCSF_MCS00_ELISA_959.jpg)
![N/A G-CSF [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_GCSF_DCS50_ELISA_637.jpg)
![N/A G-CSF [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_GCSF_DY414_ELISA_2011.jpg)
![Bioactivity G-CSF [Unconjugated]](http://images.novusbio.com/fullsize/protein/G-CSF_214-CS_424.jpg)
![Immunohistochemistry G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/fullsize/mab414_mouse-g-csf-mab-clone-67604-immunohistochemistry-121220257434724.jpg)
![Flow Cytometry G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/fullsize/mab414_mouse-g-csf-mab-clone-67604-flow-cytometry-12122025815217.jpg)
![Flow Cytometry G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/fullsize/mab414_mouse-g-csf-mab-clone-67604-flow-cytometry-12122025811814.jpg)
![Flow Cytometry G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/fullsize/mab414_mouse-g-csf-mab-clone-67604-flow-cytometry-121220258461019.jpg)
![Immunohistochemistry G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/fullsize/mab414_mouse-g-csf-mab-clone-67604-immunohistochemistry-12122025815500.jpg)
![Flow Cytometry G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/fullsize/mab414_mouse-g-csf-mab-clone-67604-flow-cytometry-121220258152612.jpg)
![ELISA G-CSF Antibody (67604) [Unconjugated]](http://images.novusbio.com/images/mab414_mouse-g-csf-mab-clone-67604-elisa-639201778464124170.jpg)
![Neutralization G-CSF Antibody [Unconjugated]](http://images.novusbio.com/images/af-214-na_human-g-csf-affinity-purified-polyclonal-ab-neutralization-639184423069245140.jpg)
![ELISA G-CSF Antibody [Unconjugated]](http://images.novusbio.com/images/af-214-na_human-g-csf-affinity-purified-polyclonal-ab-elisa-639201852913971366.jpg)
![ELISA G-CSF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/GCSF_AF414NA_ELISA_22442.jpg)
![ELISA G-CSF Antibody [Unconjugated]](http://images.novusbio.com/images/af-414-na_mouse-g-csf-affinity-purified-polyclonal-ab-elisa-639201852913983729.jpg)
![N/A G-CSF [HRP]](http://images.novusbio.com/fullsize/elisa/G-CSF_MCS00_ELISA_254.jpg)
![ELISA G-CSF [HRP]](http://images.novusbio.com/fullsize/mcs00_mouse-g-csf-quantikine-elisa-kit-41202412521112.jpg)
![N/A G-CSF [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_GCSF_DCS50_ELISA_638.jpg)
![N/A G-CSF [HRP]](http://images.novusbio.com/fullsize/elisa/G-CSF_DCS50_ELISA_70.jpg)
![SDS-Page G-CSF [Unconjugated]](http://images.novusbio.com/fullsize/protein/G-CSF_214-CS_423.jpg)
