FUS Antibody (CL0190) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line HeLa cytoplasmic fractionLane 3: Human cell line HeLa membrane fractionLane 4: Human cell line HeLa nuclear fractionLane 5: Human cell line HeLa chromatin fractionLane 6: Human cell line HeLa cytoskeletal fraction

Staining in HeLa cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Lane 1: Marker [kDa]Lane 2: Human cell line HeLaLane 3: Human cell line A-431Lane 4: Human cell line MCF-7Lane 5: Human cell line U2-OSLane 6: Human cell line Hep-G2

Staining in MCF7 cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U2OS cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U251 cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Analysis in human cerebral cortex and liver tissues using NBP3-44055 antibody. Corresponding FUS RNA-seq data are presented for the same tissues.

Staining in A431 cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human endometrium shows strong nuclear positivity in glandular cells.

Staining of human liver shows only very weak nuclear positivity in hepatocytes.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-FUS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0190
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FUS Antibody (CL0190) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse FUS Antibody (CL0190) - Azide and BSA Free (NBP2-52874) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P35637, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for FUS Antibody (CL0190) - Azide and BSA Free

• 75 kDa DNA-pairing protein
• ALS6
• amyotrophic lateral sclerosis 6
• FUS1
• fused in sarcoma
• fusion (involved in t(12;16) in malignant liposarcoma)
• fusion gene in myxoid liposarcoma
• fusion, derived from t(12;16) malignant liposarcoma
• fus-like protein
• heterogeneous nuclear ribonucleoprotein P2
• HNRNPP2
• Oncogene FUS
• Oncogene TLS
• POMP75
• RNA-binding protein FUS
• TLShnRNP-P2
• Translocated in liposarcoma protein

Background

FUS, fused in sarcoma, encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins. Involved in placental, cardiac, hepatic and embryonic development. NCOA6 is over-expressed in several breast cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUS