ASPM Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ASPM Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 3425 and the C-terminus (residue 3477) of human ASP (Abnormal Spindle)-like, Microcephaly Associated using the numbering given in entry NP_060606.2 (GeneID 259266).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ASPM
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:100 - 1:500
Application Notes
For ICC/IF: Permeabilization with Triton-X 100 is recommended. Results in tissue staining is mixed but tend to give high background. Likely to work with frozen sections. Immunohistochemistry was reported in scientific literature.
Publications
Read Publications using
NB100-2278 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22350525).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ASPM Antibody

  • asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
  • Asp homolog
  • ASP
  • Calmbp1
  • FLJ43117
  • MCPH5DKFZp686N06184
  • microcephaly associated (Drosophila)
  • microcephaly, primary autosomal recessive 5

Background

The ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002). The mouse gene Aspm is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ASPM Antibody (NB100-2278)(5)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NB100-2278

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Bioinformatics

Gene Symbol ASPM
Entrez
Uniprot