Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC ...read more
The immunogen recognized by this antibody maps to a region between residue 3425 and the C-terminus (residue 3477) of human ASP (Abnormal Spindle)-like, Microcephaly Associated using the numbering given in entry NP_060606.2 (GeneID 259266).
Immunogen affinity purified
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For ICC/IF: Permeabilization with Triton-X 100 is recommended. Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Likely to work with frozen sections. IHC was reported in scientific literature (PMID: 22350525). Use in IHC-P reported in scientific literature (PMID: 30092011).
Read Publications using NB100-2278 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 22350525).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for ASPM Antibody
asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
microcephaly associated (Drosophila)
microcephaly, primary autosomal recessive 5
The ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002). The mouse gene Aspm is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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