FoxP2 Antibody (CL5310) - Azide and BSA Free

Staining of human fallopian tube shows weak to moderate nuclear positivity in glandular cells.

Staining of human cerebral cortex shows strong nuclear positivity in deep layers neurons.

Staining of human rectum shows strong nuclear positivity in glandular cells.

Staining of human lymph node shows no positivity in non-germinal center cells as expected.

Staining of mouse brain shows strong nuclear positivity in layer 6 cortical neurons, as well as in caudatoputamen.

Staining of RH-30 cells using the Anti-FoxP2 monoclonal antibody) .

Staining of mouse cerebral cortex shows strong nuclear positivity in layer 6 neurons.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clone: CL5310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FoxP2 Antibody (CL5310) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of O15409, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for FoxP2 Antibody (CL5310) - Azide and BSA Free

• CAG repeat protein 44
• CAGH44
• CAGH44TNRC10forkhead box protein P2
• DKFZp686H1726
• forkhead box P2
• forkhead/winged-helix transcription factor
• FoxP2
• SPCH1
• TNRC10
• trinucleotide repeat containing 10
• Trinucleotide repeat-containing gene 10 protein

Background

FOXP2 encodes an evolutionarily conserved transcription factor expressed in fetal and adult brain. This transcription factor is a member of the forkhead/winged-helix (FOX) family of transcription factors, and contains a FOX DNA-binding domain and a large polyglutamine tract. Members of the FOX family of transcription factors are regulators of embryogenesis. The product of this gene is thought to be required for proper development of speech and language regions of the brain during embryogenesis. Although a point mutation in this gene has been associated with the KE pedigree segregating developmental verbal dyspraxia, no association between mutations in this gene and another speech disorder, autism, has been found. Four alternative transcripts encoding three different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FOXP2