FoxO1/FKHR [p Ser256] Antibody - BSA Free

Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.

Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunofluorescent staining in MCF7 cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FoxO1/FKHR [p Ser256] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FoxO1/FKHR [p Ser256] Antibody - BSA Free (NB100-81927) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-FoxO1/FKHR Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human FoxO1/FKHR around the phosphorylation site of serine 256.
• Modification: p Ser256
• Specificity: Detects endogenous levels of FoxO1/FKHR only when phosphorylated at serine 256.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  FoxO1/FKHR Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FoxO1/FKHR [p Ser256] Antibody - BSA Free

• FKH1
• FKHR
• FKHRforkhead box protein O1
• forkhead box O1
• Forkhead box protein O1A
• forkhead homolog in rhabdomyosarcoma
• Forkhead in rhabdomyosarcoma
• FoxO1
• FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxO1/FKHR Antibody (NB100-81927)(9)

Publications using NB100-81927

• Liu N, Li R, Cao J et al. The inhibition of FKBP5 protects ? cell survival under inflammation stress via AKT/FOXO1 signaling. Research Square 2023-02-07 (ICC/IF, WB, Human)
• Milanovi?, M;Beki?, M;?oki?, J;Vu?evi?, D;?oli?, M;Tomi?, S; Exogenous ?-ketoglutarate Modulates Redox Metabolism and Functions of Human Dendritic Cells, Altering Their Capacity to Polarise T Cell Response International journal of biological sciences 2024-01-20 [PMID: 38322117]
• Liu N, Li R, Cao J et al. The inhibition of FKBP5 protects ?-cell survival under inflammation stress via AKT/FOXO1 signaling Cell death discovery 2023-07-14 [PMID: 37452039] (WB, Mouse)
• Yang KJ, Choi WJ, Chang YK et al. Inhibition of Xanthine Oxidase Protects against Diabetic Kidney Disease through the Amelioration of Oxidative Stress via VEGF/VEGFR Axis and NOX-FoxO3a-eNOS Signaling Pathway International journal of molecular sciences 2023-02-14 [PMID: 36835220] (WB, Mouse)
• Liu Z, Du J, Ren J et al. miR-183-96-182 clusters alleviated ox-LDL-induced vascular endothelial cell apoptosis in vitro by targeting FOXO1 RSC Adv 2022-05-13 [PMID: 35547044]
• KE Ying, W Feng, WZ Ying, X Li, D Xing, Y Sun, Y Chen, PW Sanders Dietary salt initiates redox signaling between endothelium and vascular smooth muscle through NADPH oxidase 4 Redox Biology, 2022-03-24;52(0):102296. 2022-03-24 [PMID: 35378363] (Western Blot, Human)
• Miyake M, Hori S, Itami Y et al. Supplementary Oral Anamorelin Mitigates Anorexia and Skeletal Muscle Atrophy Induced by Gemcitabine Plus Cisplatin Systemic Chemotherapy in a Mouse Model Cancers (Basel) 2020-07-17 [PMID: 32709007] (WB, Mouse)
• Presby DM, Checkley LA, Jackman MR et al. Regular exercise potentiates energetically expensive hepatic de novo lipogenesis during early weight regain Am. J. Physiol. Regul. Integr. Comp. Physiol. 2019-09-25 [PMID: 31553623] (WB, Rat)
• Haapalainen AM, Karjalainen MK, Daddali R et al. Expression of CPPED1 in human trophoblasts is associated with timing of term birth. J. Cell. Mol. Med. 2017-11-29 [PMID: 29193784] (WB, Human)

Bioinformatics

• Gene Symbol: FOXO1
• Entrez:
  • 2308(Human)
  • 56458(Mouse)
  • 84482(Rat)
• Uniprot:
  • Q12778(Human)