FoxO1/FKHR Antibodies
We offer FoxO1/FKHR Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each FoxO1/FKHR Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our FoxO1/FKHR Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Primate, Rat. Use the list below to choose the FoxO1/FKHR Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our FoxO1/FKHR polyclonal antibodies and browse our FoxO1/FKHR monoclonal antibody catalog.
Alternate Names for FoxO1/FKHR Antibodies
anti-FoxO1/FKHR antibody, anti-FOXO1 antibody, anti-FKH1 antibody, anti-FKHRforkhead box protein O1 antibody, anti-forkhead box O1 antibody, anti-Forkhead box protein O1A antibody, anti-forkhead homolog in rhabdomyosarcoma antibody, anti-Forkhead in rhabdomyosarcoma antibody, anti-FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
20 Results for "foxo1-fkhr" in Primary Antibodies
NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="FoxO1/FKHR was detected in immersion fixed LNCaP human prostate cancer cell line using Human FoxO1/FKHR Monoclonal Antibody (Catalog # MAB5939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Western-Blot-NBP2-31376-img0007.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunocytochemistry-Immunofluorescence-NBP2-31376-img0009.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunohistochemistry-Paraffin-NBP2-31376-img0011.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0016.jpg)
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Western-Blot-NBP2-31376-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunohistochemistry-Paraffin-NBP2-31376-img0010.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0013.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0014.jpg)
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0015.jpg)
![Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Simple-Western-NBP2-31376-img0012.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-2342024155517.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunohistochemical staining in human lung cancer formalin fixed paraffin-embedded tissue section.")
![Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR [p Ser256] Antibody [NB100-81927] - Immunofluorescent staining in MCF7 cells.](http://images.novusbio.com/fullsize/nb100-81927_rabbit-polyclonal-foxo1-fkhr-p-ser256-antibody-234202415232510.jpg)
![Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Western-Blot-NB100-2312-img0016.jpg "Western Blot: FoxO1/FKHR Antibody [NB100-2312] - Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-FOXO1a antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FoxO1/FKHR Antibody [NB100-2312] - Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXO1a antibody NB100-2312 (lot NB100-2312-3) used for IP at 6 ug per reaction. FOXO1a was also immunoprecipitated by a previous lot of this antibody (lot NB100-2312-2). For blotting immunoprecipitated FOXO1a, NB100-2312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunoprecipitation-NB100-2312-img0015.jpg)
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0006.jpg "Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunofluorescence analysis of Hela cells using FOXO1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP2-22190-img0001.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22190-img0002.jpg)
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0004.jpg)
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0005.jpg)
![ELISA: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-ELISA-NBP2-22190-img0003.jpg)
![Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Western-Blot-NBP2-67463-img0008.jpg "Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0002.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0004.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0006.jpg)
![Western blot analysis on different lysates with NBP2-67463 at 1/5,000 dilution. Lane 1: THP-1 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: NIH/3T3 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67463 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67463_-western-blot-639204586672010352.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunohistochemistry-Paraffin-NBP1-84674-img0008.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry: FoxO1/FKHR Antibody [NBP1-84674] - Staining of mouse cerebral cortex shows moderate to strong positivity in more than 75 % of neurons in the visual cortex.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunohistochemistry-NBP1-84674-img0005.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NBP1-84674] - Staining of human stomach shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Immunohistochemistry-Paraffin-NBP1-84674-img0006.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for ![Immunocytochemistry/Immunofluorescence: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraformaldehyde-fixed HeLa, using antibody (7H3) at 1:100 dilution.](http://images.novusbio.com/fullsize/FOXO1A-Antibody-7H3-Immunocytochemistry-Immunofluorescence-NB200-307-img0001.jpg "Immunocytochemistry/Immunofluorescence: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraformaldehyde-fixed HeLa, using antibody (7H3) at 1:100 dilution.")
![Immunohistochemistry-Paraffin: FOXO1A Antibody (7H3) [NB200-307] - Analysis of paraffin-embedded Colon ca, using antibody (7H3) at 1:50 dilution.](http://images.novusbio.com/fullsize/FOXO1A-Antibody-7H3-Immunohistochemistry-Paraffin-NB200-307-img0004.jpg)
![FoxO1/FKHR antibody [7H3] detects FoxO1/FKHR protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human cervix.</br>FoxO1/FKHR stained by FoxO1/FKHR antibody [7H3] (NB200-307) diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nb200-307_mouse-monoclonal-foxo1-fkhr-antibody-7h3-immunohistochemistry-paraffin-141120251834153.jpg)
![Western Blot: FoxO1/FKHR Antibody [NBP2-24857] - Analysis of FOXO1A in human ovary lysate in the (1) absence and (2) presence of immunizing peptide using this antibody at 0.05 ug/ml.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Western-Blot-NBP2-24857-img0002.jpg "Western Blot: FoxO1/FKHR Antibody [NBP2-24857] - Analysis of FOXO1A in human ovary lysate in the (1) absence and (2) presence of immunizing peptide using this antibody at 0.05 ug/ml.")
![Western Blot: FoxO1/FKHR Antibody [NB100-81924] - Analysis of extracts from Hela cells using NB100-81924](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-Western-Blot-NB100-81924-img0001.jpg "Western Blot: FoxO1/FKHR Antibody [NB100-81924] - Analysis of extracts from Hela cells using NB100-81924")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NB100-81924] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81924(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-81924_rabbit-polyclonal-foxo1-fkhr-antibody-103202523464521.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody [NB100-81924] - Staining of methanol-fixed Hela cells using NB100-81924](http://images.novusbio.com/fullsize/nb100-81924_rabbit-polyclonal-foxo1-fkhr-antibody-11320251555913.jpg)
