FoxO1/FKHR Antibody (83N7F8) [Alexa Fluor® 488]

Images

 
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [Alexa Fluor 488] [NBP2-31376AF488] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining ...read more
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [Alexa Fluor 488] [NBP2-31376AF488] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, SqSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
83N7F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

FoxO1/FKHR Antibody (83N7F8) [Alexa Fluor® 488] Summary

Immunogen
FOXO1A
Localization
Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus.
Predicted Species
Mouse (92%), Rat (92%), Porcine (90%), Bovine (91%), Squirrel (91%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOXO1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Reviewed Applications
Read 1 Review rated 4
using
NBP2-31376AF488 in the following applications:

Reactivity Notes

Human. Based upon immunogen sequence simlarity, this antibody is predicted to react with Mouse (92%), Rat (92%), Bovine/Cow (91%), Squirrel (91%) and Porcine/Pig (90%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for FoxO1/FKHR Antibody (83N7F8) [Alexa Fluor® 488]

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxO1/FKHR Antibody (NBP2-31376AF488) (0)

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Review for FoxO1/FKHR Antibody (NBP2-31376AF488) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-31376AF488:
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Flow
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry FoxO1/FKHR NBP2-31376AF488
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reviewed by:
Anonymous
Flow Human 06/17/2016
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Summary

ApplicationFlow Cytometry
Sample TestedPrimary CD4+ T-cells isolated from peripheral blood.
SpeciesHuman

Blocking

Blocking DetailsNo blocking was performed.

Primary Anitbody

Dilution Ratio1uL of antibody per 10e5 cells; 45 minutes incubation at room temperature; Permeabilization buffer from eBioscience (00-5523-00)

Details

Detection NotesThe Attune NxT flow cytometer was used for acquisition; the FlowJo software was used for analysis.
Permeabilization DetailsIntracellular Staining Buffer set (eBioscience, San Diego, CA; cat. 00-5523-00); manufacture's instructions.
Wash DescriptionPermeabilization buffer from eBioscience (cat. 00-5523-00).

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NBP2-31376AF488) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FoxO1/FKHR Antibody (NBP2-31376AF488)

Discover related pathways, diseases and genes to FoxO1/FKHR Antibody (NBP2-31376AF488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FoxO1/FKHR Antibody (NBP2-31376AF488)

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PTMs for FoxO1/FKHR Antibody (NBP2-31376AF488)

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Blogs on FoxO1/FKHR.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Recent Reviews

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Anonymous
06/17/2016
Application: Flow
Species: Human

Bioinformatics

Gene Symbol FOXO1
Entrez