FoxJ1/HFH4 Antibody [Unconjugated]

HAF017). A specific band was detected for FoxJ1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for FoxJ1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxJ1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="FoxJ1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

FoxJ1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." title="FoxJ1 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." />

FoxJ1 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." > HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." title="Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." />

Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FoxJ1/HFH4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FoxJ1
  Lys306-Leu421
  Accession # Q92949
• Specificity: Detects human FoxJ1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXJ1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxJ1/HFH4 Antibody [Unconjugated]

• FKHL13
• FKHL13forkhead transcription factor HFH-4
• forkhead box J1
• forkhead-like 13
• Forkhead-related protein FKHL13
• FoxJ1
• Hepatocyte nuclear factor 3 forkhead homolog 4
• HFH-4
• HFH4fork head homologue 4
• HFH-4forkhead box protein J1
• MGC35202

Background

FoxJ1 (Forkhead box protein J1; also HFH4) is a 55-65 kDa, class 1 member of the HNF-3/forkhead gene family of transcription factors. Its observed MW is higher than its predicted MW (45 kDa), suggesting anomalous migration on SDS-PAGE. FoxJ1 promotes the expression of cilia in respiratory, oviduct and choroid plexus epithelium, and maintains T cell tolerance to self-antigens. Human FoxJ1 is 421 amino acids (aa) in length, and contains one forkhead DNA binding domain (aa's 120‑210). The presence of basic residues in the forkhead domain makes FoxJ1 a class 1 Fox protein. There is potential splice variant that contains a 25 aa substation for aa 262-287. Over aa 306-421, human FoxJ1 shares 88% aa with mouse FoxJ1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxJ1/HFH4 Antibody (AF3619)(10)

Publications using AF3619

• Wei, H;Wu, X;Withrow, J;Cuevas-Diaz Duran, R;Singh, S;Chaboub, LS;Rakshit, J;Mejia, J;Rolfe, A;Herrera, JJ;Horner, PJ;Wu, JQ; Glial progenitor heterogeneity and key regulators revealed by single-cell RNA sequencing provide insight to regeneration in spinal cord injury Cell reports 2023-05-06 [PMID: 37149868] (Immunohistochemistry, Transgenic Mouse)
• Shunya Tsuji, Shohei Minami, Rina Hashimoto, Yusuke Konishi, Tatsuya Suzuki, Tamae Kondo et al. SARS-CoV-2 infection triggers paracrine senescence and leads to a sustained senescence-associated inflammatory response Nature Aging 2022-01-25 [PMID: 37117754] (Immunocytochemistry, Human)
• Chen M, Pekosz A, Villano JS et al. Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants bioRxiv : the preprint server for biology 4/12/2022 [PMID: 35441175]
• IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional Nature Communications, 2021-07-27;12(1):4566. 2021-07-27 [PMID: 34315881] (IHC, Human)
• R Robinot, M Hubert, GD de Melo, F Lazarini, T Bruel, N Smith, S Levallois, F Larrous, J Fernandes, S Gellenonco, S Rigaud, O Gorgette, C Thouvenot, C Trébeau, A Mallet, G Duménil, S Gobaa, R Etournay, PM Lledo, M Lecuit, H Bourhy, D Duffy, V Michel, O Schwartz, LA Chakrabart SARS-CoV-2 infection induces the dedifferentiation of multiciliated cells and impairs mucociliary clearance Nature Communications, 2021-07-16;12(1):4354. 2021-07-16 [PMID: 34272374] (ICC, Human)
• LN Manganas, I Durá, S Osenberg, F Semerci, M Tosun, R Mishra, L Parkitny, JM Encinas, M Maletic-Sa BASP1 labels neural stem cells in the neurogenic niches of mammalian brain Scientific Reports, 2021-03-10;11(1):5546. 2021-03-10 [PMID: 33692421] (IHC, Mouse)
• MN Ali, M Mori, TCJ Mertens, P Siddhuraj, JS Erjefält, P Önnerfjord, PS Hiemstra, A Egesten Osteopontin Expression in Small Airway Epithelium in Copd is Dependent on Differentiation and Confined to Subsets of Cells Sci Rep, 2019-10-29;9(1):15566. 2019-10-29 [PMID: 31664154] (IHC, Human)
• Gefitinib, an EGFR Tyrosine Kinase inhibitor, Prevents Smoke-Mediated Ciliated Airway Epithelial Cell Loss and Promotes Their Recovery PLoS ONE, 2016-08-17;11(8):e0160216. 2016-08-17 [PMID: 27532261] (IHC-P, Human)
• Gerovac BJ, Valencia M, Baumlin N et al. Submersion and Hypoxia Inhibit Ciliated Cell Differentiation in a Notch Dependent Manner.. Am. J. Respir. Cell Mol. Biol. [PMID: 24754775]
• Casalino-Matsuda SM, Monzon ME, Day AJ, Forteza RM Hyaluronan fragments/CD44 mediate oxidative stress-induced MUC5B up-regulation in airway epithelium. Am. J. Respir. Cell Mol. Biol., 2008-08-28;40(3):277-85. 2008-08-28 [PMID: 18757307] (IHC-P, Human)

Bioinformatics

• Gene Symbol: FOXJ1
• Uniprot:
  • Q92949()