FoxJ1/HFH4 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

FoxJ1/HFH4 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human FoxJ1
  Lys306-Leu421
  Accession # Q92949
• Specificity: Detects human FoxJ1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxJ1/HFH4 Antibody [Alexa Fluor® 488]

• FKHL13
• FKHL13forkhead transcription factor HFH-4
• forkhead box J1
• forkhead-like 13
• Forkhead-related protein FKHL13
• FoxJ1
• Hepatocyte nuclear factor 3 forkhead homolog 4
• HFH-4
• HFH4fork head homologue 4
• HFH-4forkhead box protein J1
• MGC35202

Background

FoxJ1 (Forkhead box protein J1; also HFH4) is a 55-65 kDa, class 1 member of the HNF-3/forkhead gene family of transcription factors. Its observed MW is higher than its predicted MW (45 kDa), suggesting anomalous migration on SDS-PAGE. FoxJ1 promotes the expression of cilia in respiratory, oviduct and choroid plexus epithelium, and maintains T cell tolerance to self-antigens. Human FoxJ1 is 421 amino acids (aa) in length, and contains one forkhead DNA binding domain (aa's 120‑210). The presence of basic residues in the forkhead domain makes FoxJ1 a class 1 Fox protein. There is potential splice variant that contains a 25 aa substation for aa 262-287. Over aa 306-421, human FoxJ1 shares 88% aa with mouse FoxJ1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.