FOXG1 Antibody - Azide and BSA Free

Images

 
Western Blot: FOXG1 Antibody [NBP3-04084] - Analysis of extracts of various cell lines, using FOXG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: ...read more
Immunocytochemistry/ Immunofluorescence: FOXG1 Antibody [NBP3-04084] - Analysis of U2OS cells using FOxG1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

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Catalog# & Formulation Size Price

FOXG1 Antibody - Azide and BSA Free Summary

Description
Novus Biologicals Rabbit FOXG1 Antibody - Azide and BSA Free (NBP3-04084) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-489 of human FOXG1 (NP_005240.3). YFTHQNQGSSSNPLIH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXG1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Western Blot 1:500 - 1:1000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP3-04084 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for FOXG1 Antibody - Azide and BSA Free

  • BF-1
  • BF1HBF-2
  • BF2
  • BF-2
  • Brain factor 1
  • Brain factor 2
  • FHKL3
  • FKHL1forkhead-like 1
  • FKHL2
  • FKHL3
  • FKHL4
  • forkhead box G1
  • forkhead box G1A
  • forkhead box G1B
  • forkhead box G1C
  • Forkhead box protein G1A
  • Forkhead box protein G1B
  • Forkhead box protein G1C
  • forkhead-like 3
  • forkhead-like 4
  • Forkhead-related protein FKHL1
  • Forkhead-related protein FKHL2
  • Forkhead-related protein FKHL3
  • FOXG1A
  • FOXG1Bforkhead-like 2
  • FOXG1C
  • HBF-1
  • hBF-2
  • HBF-3
  • HFK1HBF-G2
  • HFK2HBF2
  • HFK3KHL2
  • oncogene QIN
  • QINFKH2forkhead box protein G1

Background

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. This gene participates in signal transduction Activin A signaling regulation, TGF-beta receptor signaling pathway, transcription factors in neurogenesis, as well as regulation of nuclear SMAD2/3 signaling. It is thought to interact with genes FOXO1, FOXO3, FOXO4, SMAD2, and SMAD3. FOXG1 is associated with diseases such as melanoma, neuronitis, glioblastoma, purpura, west syndrome, rett syndrome, medulloblastoma, hypotonia, retinitis, maternal uniparental disomy, chromosome 14, intellectual disabilities, brain malformations, multiple sclerosis, and lupus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Publications for FOXG1 Antibody (NBP3-04084) (0)

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Review for FOXG1 Antibody (NBP3-04084) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP3-04084:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot FOXG1 NBP3-04084
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5
reviewed by:
Verified Customer
WB Mouse 11/29/2022
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Summary

ApplicationWestern Blot
Sample TestedMouse brain
SpeciesMouse
Lot1166210101

Comments

CommentsBlock: 5% nonfat milk o/n 4 C
FOXG1 antibody 1:1000 (1h RT or o/n 4 C work equally well)
Secondary: 1:80,000 Sigma goat anti-rabbit (1h RT)
BioRad Clarity ECL, imaged on Azure Biosystems 300 imager
In some samples, an additional band shows up ~70 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FOXG1 Antibody (NBP3-04084) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for FOXG1 Antibody (NBP3-04084)

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Blogs on FOXG1.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

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Recent Reviews

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1
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Verified Customer
11/29/2022
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FOXG1