FoxC2 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

FoxC2 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse FoxC2
  Ala412-Tyr494
  Accession # Q61850
• Specificity: Detects mouse FoxC2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) FoxC2 is observed and less than 3% cross-reactivity with rhFoxF2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxC2 Antibody [Alexa Fluor® 647]

• Fkh14
• FKHL14LD
• forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• forkhead, Drosophila, homolog-like 14
• Forkhead-related protein FKHL14
• FoxC2
• LD
• Mesenchyme fork head protein 1
• mesenchyme forkhead 1
• MFH-1 protein
• MFH1
• MFH-1
• MFH1forkhead box protein C2
• Transcription factor FKH-14

Background

FOXC2 (Forkhead box protein C2; also BF-3, FKH-14 and MFH-1) is a 64-66 kDa member of the winged helix transcription factor gene family. It is widely expressed, including in endothelium where it induces the appearance of CXCR4 and integrin beta 3, two molecules essential to cell migration. High-calorie diets also induce FOXC2 in adipocytes, inducing a brown-fat like phenotype. Mouse FOXC2 is 494 amino acids (aa) in length. It contains a forkhead DNA binding domain (aa 71-148), a short poly-Arg segment (aa 162-166), one His-rich region (aa 386-395) and an Ala/Pro-rich domain (aa 396-415). There are seven potential Ser/Thr phosphorylation sites. Over aa 412-494, mouse FOXC2 shares 99% and 87% aa identity with rat and human FOXC2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.