Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

Western Blot: Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) [NBP2-12311] - Lysates of human placental tissue.

Product Discontinued

Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free Summary

• Immunogen: Human recombinant Flt-3/Flk-2/CD135
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FLT3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:100
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/mL.

Alternate Names for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

• CD135 antigen
• CD135
• EC 2.7.10
• fetal liver kinase 2
• FL cytokine receptor
• FLK2
• Flk-2
• FLT3 receptor tyrosine kinase
• Flt3
• Flt-3
• Fms-like tyrosine kinase 3
• fms-related tyrosine kinase 3
• growth factor receptor tyrosine kinase type III
• Stem cell tyrosine kinase 1
• STK-1
• STK1EC 2.7.10.1
• Tyrosine-protein kinase receptor FLT3

Background

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flt-3/Flk-2/CD135 Antibody (NBP2-12311)(1)

Publication using NBP2-12311

• Karbowski, C, Goldstein, R Et al. Nonclinical Safety Assessment of AMG 553, an Investigational Chimeric Antigen Receptor T-Cell Therapy for the Treatment of Acute Myeloid Leukemia. Toxicol Sci 2020-09-01 [PMID: 32589753] (WB, Human)

FAQs for Flt-3/Flk-2/CD135 Antibody (NBP2-12311). (Showing 1 - 1 of 1 FAQ).

1. We have purchased your Flt3/CD135 Antibody (MM0294-9G23) cat# NBP2-12311 (0.1 mg lyophilized in PBS). The reconstitution instructions on the data sheet say Please contact technical support for detailed reconstitution instructions. Can you please give me the instructions how I should correctly resuspend this reagent? Also, can you confirm that this reagent is carrier free and is only the Ab in PBS (i.e. no BSA)?
   • You should spin down the vial and reconstitute in 200uL of PBS. This antibody contains no carrier proteins.

Bioinformatics

• Gene Symbol: FLT3
• Uniprot:
  • P36888()