Flotillin-1 Antibody - BSA Free

Immunoprecipitation: Flotillin-1 Antibody [NBP2-16508] - IP samples: HCT-116 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 ug Flotillin 1 antibody [C3], C-term 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Western Blot: Flotillin-1 Antibody [NBP2-16508] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Ha, Po, Bv, Pm, RM
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Flotillin-1 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin-1. The exact sequence is proprietary.
• Localization: Cell membrane; Peripheral membrane protein; Membrane; caveola; Peripheral membrane protein; Melanosome
• Predicted Species: Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLOT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Simple Western 1:20
  • Western Blot 1:500-1:3000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Extracted HER2+ Breast Cancer Donor FFPE and Healthy Donor FFPE as samples; separated by size; antibody dilution of 1:20; matrix was 12-230 kDa; detected by Chemiluminescence.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Flotillin-1 Antibody - BSA Free

• flotillin 1
• flotillin-1
• integral membrane component of caveolae

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FLOT1
• Uniprot:
  • O75955(Human)