Flotillin 2 Antibody

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug mouse brain lysate/extract. Antibody dilution 1:1000.

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000.

Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881] - Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 ug of Flotillin 2 antibody [C3], C-term. Western blot analysis was performed using Flotillin 2 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Flotillin 2 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.
• Localization: Cell membrane, Peripheral membrane protein, Membrane, caveola, Peripheral membrane protein
• Predicted Species: Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLOT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Flotillin 2 Antibody

• Epidermal surface antigen
• ESA1Membrane component chromosome 17 surface marker 1
• ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1)
• flotillin 2

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for Flotillin 2 Antibody (NBP1-30881) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Nora Mellouk WB Human 10/11/2019

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: 39443

Bioinformatics

• Gene Symbol: FLOT2
• Entrez:
  • 2319(Human)
• Uniprot:
  • Q14254(Human)