Flotillin-1 Antibody - BSA Free

Western Blot: Flotillin-1 Antibody [NBP1-79022] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.

Simple Western: Flotillin-1 Antibody [NBP1-79022] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Flotillin-1 at approximately 51 kDa (as indicated) using 1:250 ug/mL of Flotillin-1 antibody (NBP1-79022). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Po, Bv, Pm, Pm-Or
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Flotillin-1 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human Flotillin 1 using the numbering given in entry NP_005794.1 (GeneID 10211).
• Predicted Species: Porcine (100%), Chimpanzee (100%), Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FLOT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg
  • Simple Western
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Flotillin-1 Antibody - BSA Free

• flotillin 1
• flotillin-1
• integral membrane component of caveolae

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flotillin-1 Antibody (NBP1-79022)(3)

Publications using NBP1-79022

• Eitan E, Thornton-Wells T, Elgart K et al. Synaptic proteins in neuron-derived extracellular vesicles as biomarkers for Alzheimer's disease: novel methodology and clinical proof of concept Extracellular Vesicles and Circulating Nucleic Acids 2023-03-31 (IA, Human)
• Brambilla D, Panico F, Unlu M, Chiari M Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates ChemRxiv 2023-11-29 (WB, Human)
• Lu A, Wawro P, Morgens DW et al. Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs Elife 2018-12-17 [PMID: 30556811] (Human)

Review for Flotillin-1 Antibody (NBP1-79022) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 09/06/2017

Summary

• Application: Immunofluorescence
• Sample Tested: Cortical neurons
• Species: Mouse
• Lot: A1

Bioinformatics

• Gene Symbol: FLOT1
• Uniprot:
  • NP_005794.1(Human)