Ficolin-1 Overexpression Lysate

Images

 
Western Blot: FCN1 Overexpression Lysate (Adult Normal) [NBL1-10662] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FCN1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Ficolin-1 Overexpression Lysate Summary

Description
Transient overexpression lysate of ficolin (collagen/fibrinogen domain containing) 1 (FCN1) The lysate was created in HEK293T cells, using Plasmid ID RC204637 and based on accession number NM_002003. The protein contains a C-MYC/DDK Tag.
Gene
FCN1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
32.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Ficolin-1 Overexpression Lysate

  • Collagen/fibrinogen domain-containing protein 1
  • FCN1
  • Fcna
  • FCNM
  • FCNMficolin-A
  • ficolin (collagen/fibrinogen domain containing) 1
  • ficolin (collagen/fibrinogen domain-containing) 1
  • Ficolin1
  • Ficolin-1
  • Ficolin-A
  • ficolin-alpha
  • M-ficolin

Background

The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Ficolin-1 Lysate (NBL1-10662) (0)

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Other Available Formats

NBL1-10662

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Bioinformatics

Gene Symbol FCN1