Fibulin 5/DANCE Antibody (1G6A4)

Western Blot: Fibulin 5/DANCE Antibody (1G6A4) [NBP1-28890] - Analysis using FBLN5 mouse mAb against truncated FBLN5-His recombinant protein.

Immunohistochemistry-Paraffin: Fibulin 5/DANCE Antibody (1G6A4) [NBP1-28890] - Analysis of human colon cancer (left) and breast cancer (right) showing cytoplasmic localization with DAB staining using FBLN5 mouse mAb.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1G6A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Fibulin 5/DANCE Antibody (1G6A4) Summary

• Description: Novus Biologicals Mouse Fibulin 5/DANCE Antibody (1G6A4) (NBP1-28890) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of Fibulin 5/DANCE (aa242-448) expressed in E. Coli.
• Specificity: Specific for FBLN5.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBLN5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1: 10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Fibulin 5/DANCE Antibody (1G6A4)

• ARMD3fibulin-5
• DANCE
• DANCEFIBL-5
• Developmental arteries and neural crest EGF-like protein
• EVEC
• FBLN5
• Fibulin 5
• UP50
• UP50FLJ90059
• Urine p50 protein

Background

The protein is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBLN5