Fibulin-3/EFEMP1 Antibody

Images

 
Western Blot: EFEMP1 Antibody [NBP2-16277] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Fibulin-3/EFEMP1 Antibody [NBP2-16277] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EFEMP1 protein stained by EFEMP1 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: EFEMP1 Antibody [NBP2-16277] - Immunohistochemical analysis of paraffin-embedded Colon CA, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Fibulin-3/EFEMP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human EFEMP1. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFEMP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Porcine (89%), Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fibulin-3/EFEMP1 Antibody

  • DHRD
  • EFEMP1
  • EGF containing fibulin-like extracellular matrix protein 1
  • EGF-containing fibulin-like extracellular matrix protein 1
  • Extracellular protein S1-5
  • FBLN3
  • FBLN3DRAD
  • FBNLFLJ35535
  • FIBL-3
  • Fibrillin-like protein
  • fibrillin-like
  • Fibulin 3
  • fibulin-3
  • MGC111353
  • MLVT
  • MTLV
  • S1-5

Background

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Alternative splice patterns in the 5' UTR result in three transcript variants encoding the same extracellular matrix protein. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EFEMP1
Entrez
Uniprot