Fibulin-3/EFEMP1 Antibody [Alexa Fluor® 750]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Fibulin-3/EFEMP1 Antibody [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Fibulin 3
  Gln19-Phe493
  Accession # Q12805
• Specificity: Detects human Fibulin 3 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Fibulin 3 (also S1-5 and EFEMP1) is a secreted, monomeric 55‑57 kDa member of the fibulin family of proteins. In mouse, Fibulin 3 appears in an O-glycosylated 63 and 80 kDa form. Given the high amino acid (aa) conservation between mouse and human, human Fibulin 3 may also demonstrate like isoforms. Fibulin 3 is expressed by fibroblasts, endothelial cells and prechondrocytes, and appears to provide both stability to ECM (elastin and basement membrane), and to regulate cell maturation/ differentiation. Fibulin 3 is reported to activate the EGF receptor, an effect that may influence multiple cell types under multiple scenarios. Mature human Fibulin 3 is 476 aa in length. It contains six EGF-like domains (aa 26‑378), at least one of which binds to the EGF receptor and induces EGFR phosphorylation. There is at least one alternative start site at Met107 that generates a 43 kDa short form. Full-length human Fibulin 3 shares 95% aa identity with mouse Fibulin 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.