![Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM539) [NBP2-32848] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (SPM539).](http://images.novusbio.com/fullsize2/Fibronectin-Antibody-(SPM539)-Immunohistochemistry-Paraffin-NBP2-32848-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (SPM539) [NBP2-32848] - Formalin-fixed, paraffin-embedded human Pancreatic Adeno Carcinoma stained with Fibronectin Monoclonal Antibody (SPM539).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP |
| Clone | SPM539 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose |
| Localization | Connective tissue matrix |
| Specificity | Fibronectin is a dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FN1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA (For coating, order NBP2-34785 (BSA free version of NBP2-32848)) |
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| Control |
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| Storage | Store at 4C. |
| Buffer | PBS with 0.05% BSA. |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 405 | NBP2-34785AF405 | |
| Alexa Fluor 488 | NBP2-34785AF488 | |
| Alexa Fluor 647 | NBP2-34785AF647 | |
| Alexa Fluor 700 | NBP2-34785AF700 | |
| Allophycocyanin | NBP2-34785APC | |
| Biotin | NBP2-34785B | |
| DyLight 350 | NBP2-34785UV | |
| DyLight 405 | NBP2-34785V | |
| DyLight 488 | NBP2-34785G | |
| DyLight 550 | NBP2-34785R | |
| DyLight 650 | NBP2-34785C | |
| DyLight 680 | NBP2-34785FR | |
| DyLight 755 | NBP2-34785IR | |
| HRP | NBP2-34785H | |
| Janelia Fluor 549 | NBP2-34785JF549 | |
| Janelia Fluor 646 | NBP2-34785JF646 | |
| PE | NBP2-34785PE | |
| PerCP | NBP2-34785PCP |
Diseases for Fibronectin Antibody (NBP2-32848)Discover more about diseases related to Fibronectin Antibody (NBP2-32848).
| Pathways for Fibronectin Antibody (NBP2-32848)View related products by pathway.
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![Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serum](http://images.novusbio.com/fullsize2/Vitronectin Antibody (ST49-02)-Western Blot-NBP2-67511-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Vitronectin-Antibody-(ST49-02)-Immunocytochemistry-Immunofluorescence-NBP2-67511-img0004.jpg)
![Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitronectin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Vitronectin-Antibody-(ST49-02)-Immunohistochemistry-Paraffin-NBP2-67511-img0008.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize2/FAK-Antibody-(SR46-04)-Western-Blot-NBP2-67327-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/FAK-Antibody-(SR46-04)-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg)
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/FAK Antibody (SR46-04)-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg)
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize2/Tenascin-C-Antibody-(4C8MS)-Flow-(Intracellular)-NB110-68136-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Tenascin-C-Antibody-(4C8MS)-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize2/Tenascin-C-Antibody-(4C8MS)-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - Integrin beta 1 (P4C10) antibody was tested in -20 degree MeOH fixed HeLa cells at a 1:200 dilution against DyLight 488 (green). Actin and nuclei were counterstained against Phalloidin 568 (red) and DAPI (blue), respectively.](http://images.novusbio.com/fullsize2/Integrin-beta-1-CD29-Antibody-(P4C10)-Immunocytochemistry-Immunofluorescence-NBP2-36561-img0001.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/Integrin-beta-1-CD29-Antibody-(P4C10)-Flow-Cytometry-NBP2-36561-img0004.jpg)
![Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [NBP2-36561]](http://images.novusbio.com/fullsize2/Integrin-beta-1-CD29-Antibody-(P4C10)-Flow-Cytometry-NBP2-36561-img0002.jpg)
![Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12](http://images.novusbio.com/fullsize2/ERK2 Antibody (SZ25-01)-Western Blot-NBP2-67360-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/ERK2-Antibody-(SZ25-01)-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/ERK2-Antibody-(SZ25-01)-Immunohistochemistry-Paraffin-NBP2-67360-img0007.jpg)
![Western Blot: Plasminogen Antibody [NB600-930] - Lane 1: Plasminogen. Lane 2: None. Load: 50 ng per lane. Primary antibody: Plasminogen primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 91 kDa, 91 kDa for Plasminogen. Other band(s): None.](http://images.novusbio.com/fullsize2/Plasminogen-Antibody-Western-Blot-NB600-930-img0006.jpg)
![Western Blot: Plasminogen Antibody [NB600-930] - Detection of Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize2/Plasminogen-Antibody-Western-Blot-NB600-930-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize2/CD44-Antibody-(8E2F3)-Immunocytochemistry-Immunofluorescence-NBP1-47386-img0011.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.](http://images.novusbio.com/fullsize2/CD44-Antibody-(8E2F3)-Immunohistochemistry-Paraffin-NBP1-47386-img0014.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/fullsize2/CD44-Antibody-(8E2F3)-Flow-Cytometry-NBP1-47386-img0008.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/CTGF-CCN2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-724-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Von Willebrand Factor Antibody [NB600-586]](http://images.novusbio.com/fullsize2/Von-Willebrand-Factor-Antibody-Immunocytochemistry-Immunofluorescence-NB600-586-img0003.jpg)
![Immunohistochemistry-Paraffin: Von Willebrand Factor Antibody [NB600-586] - Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor.](http://images.novusbio.com/fullsize2/Von-Willebrand-Factor-Antibody-Immunohistochemistry-Paraffin-NB600-586-img0005.jpg)
![Immunohistochemistry: Von Willebrand Factor Antibody [NB600-586] - Formalin fixed paraffin embedded human tonsil stained with Factor VIII antibody.](http://images.novusbio.com/fullsize2/Von-Willebrand-Factor-Antibody-Immunohistochemistry-NB600-586-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. Image from verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0004.jpg)
![Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. Image from verified customer review.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-Frozen-NB300-223-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0001.jpg)
![Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.](http://images.novusbio.com/fullsize2/ERK1-Antibody-(1E5)-Western-Blot-NBP2-22203-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/ERK1-Antibody-(1E5)-Immunocytochemistry-Immunofluorescence-NBP2-22203-img0002.jpg)
![Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/ERK1-Antibody-(1E5)-Immunohistochemistry-Paraffin-NBP2-22203-img0004.jpg)
![Flow Cytometry: Mouse, Human IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.](http://images.novusbio.com/images2/Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1)-Flow Cytometry-NBP1-43319-img0004.jpg "Flow Cytometry: Mouse, Human IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Mouse IgG1 Kappa Light Chain Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Involucrin signal in human keratinocytes. Red: anti-IVL antibody. BLACK: Isotype control NBP1-43319APC at 1:100. Image from verified customer review.")