Fibronectin Antibody (P1H11) [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) ...read more
Fibronectin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining; left panel) and Daudi human Burkitt's lymphoma cell line (negative staining; right panel) using Mouse ...read more
Fibronectin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Fibronectin Monoclonal Antibody (Catalog # MAB1918) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP, ICC/IF
Clone
P1H11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
View Available Formulations

Fibronectin Antibody (P1H11) [Unconjugated] Summary

Immunogen
Human plasma-derived Fibronectin
Specificity
Detects human Fibronectin in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 2 Reviews rated 4
using
MAB1918 in the following applications:

Publications
Read Publications using
MAB1918 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibronectin Antibody (P1H11) [Unconjugated]

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin is an extracellular matrix component that exists in different alternately spliced isoforms. Fibronectin mediates cell adhesion in its insoluble state but not as a soluble molecule. Fibronectins play a role in cell adhesion, migration, differentiation, and specific gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB1918
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Applications: WB, Simple Western, IHC, IP, ICC/IF

Publications for Fibronectin Antibody (MAB1918)(7)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
IHC-P
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
Canine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB1918 Applications Species
J Koch, D Mönch, A Maa beta , A Mangold, M Gužvi?, T Mürdter, T Leibold, MH Dahlke, P Renner Pharmacologic Targeting of MMP2/9 Decreases Peritoneal Metastasis Formation of Colorectal Cancer in a Human Ex Vivo Peritoneum Culture Model Cancers, 2022;14(15):. 2022 [PMID: 35954423] (IHC, Human) IHC Human
DJ Kim, JM Kang, SH Park, HK Kwon, SJ Song, H Moon, SM Kim, JW Seo, YH Lee, YG Kim, JY Moon, SY Lee, Y Son, SH Lee Diabetes Aggravates Post-ischaemic Renal Fibrosis through Persistent Activation of TGF-?1 and Shh Signalling Sci Rep, 2017;7(1):16782. 2017 [PMID: 29196746] (Western Blot, Human) Western Blot Human
Zhu W, Zhang Q, Zhang Y, Cen L, Wang J PDL regeneration via cell homing in delayed replantation of avulsed teeth. J Transl Med, 2015;13(0):357. 2015 [PMID: 26572489] (IHC-P, Canine) IHC-P Canine
Hendel , Alon, Hsu , Ivy, Granville , David J Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability. Lab Invest, 2014;94(7):716-25. 2014 [PMID: 24791744] (Western Blot, Human) Western Blot Human
Martinez-Vinambres E, Garcia-Trujillo JA, Rodriguez-Martin E, Villar LM, Coll J, Roldan E CD29 expressed on plasma cells is activated by divalent cations and soluble CD106 contained in the bone marrow plasma: refractory activation is associated with enhanced proliferation and exit of clonal plasma cells to circulation in multiple myeloma patients. Leukemia, 2011;26(5):1098-105. 2011 [PMID: 22134715] (Neutralization, Human) Neutralization Human
Li H, Wang H, Wang F, Gu Q, Xu X Snail involves in the transforming growth factor beta1-mediated epithelial-mesenchymal transition of retinal pigment epithelial cells. PLoS ONE, 2011;6(8):e23322. 2011 [PMID: 21853110] (Western Blot, Human) Western Blot Human
Hergeth SP, Aicher WK, Essl M, Schreiber TD, Sasaki T, Klein G Characterization and functional analysis of osteoblast-derived fibulins in the human hematopoietic stem cell niche. Exp. Hematol., 2008;36(8):1022-34. 2008 [PMID: 18468769] (ICC, Human) ICC Human

Reviews for Fibronectin Antibody (MAB1918) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB1918:
Filter by Applications
WB
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
cynthia morimoto
WB Human 02/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22321604
SpeciesHuman
  4
reviewed by:
cynthia morimoto
ELISA Human 02/12/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 22225866
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Fibronectin Antibody (MAB1918) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Fibronectin Antibody (MAB1918)

Discover related pathways, diseases and genes to Fibronectin Antibody (MAB1918). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Fibronectin Antibody (MAB1918)

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Pathways for Fibronectin Antibody (MAB1918)

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Blogs on Fibronectin.

Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
cynthia morimoto
02/12/2015
Application: WB
Species: Human

 
cynthia morimoto
02/12/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol FN1