Fibronectin Antibody (P1H11) [Fluorescein]

HepG2 (filled histogram) and Jurkat (open histogram) cells were stained with Mouse Anti-Human Fibronectin Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1918F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

WS-1 human fetal skin fibroblast cell line was stained with Mouse Anti-Human Fibronectin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1918F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: P1H11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

Fibronectin Antibody (P1H11) [Fluorescein] Summary

• Immunogen: Human plasma-derived Fibronectin
• Specificity: Detects human Fibronectin in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FN1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibronectin Antibody (P1H11) [Fluorescein]

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Human Fibronectin (FN) is a 210-220 kDa extracellular matrix protein that serves as a cornerstone for cell-matrix interactions. It is a sulfated glycoprotein 2055 amino acids (aa) in length. Along its length, it exhibits three types of repeating modules; twelve type I, two type II and 16 type III modules. There are more than 15 alternative splice variants, most in the C-terminal half of the molecule. FN circulates as a disulfide-linked homodimer, a product of hepatocytes. It also exists as a very large covalent multimer, likely the product of a wide variety of cell types. Within these multimers will be found FN-Fibrinogen, -Collagen 13 and -Mac2BP complexes. The multidomain nature of FN allows for binding of Fibrin, GAGs (HS and CS), Syndecans and Integrins alpha 5 beta 1 and alpha V beta 3. Over aa 31-2386 (full-length), human FN shares 91% aa sequence identity with mouse FN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FN1